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Grape skin transcriptome in the berries cultured in vitro treated with exogenous abscisic acid

ABSTRACT: The softened green berries of Cabernet Sauvignon were cultured in a Petri dish. 0.3M sucrose solution with (±)-abscisic acid (ABA) and without (±)-ABA (control) were applied to the Petri dishes. The berry skins were sampled at 3 and 10 days after the application. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV17 at PLEXdb.

ORGANISM(S): Vitis vinifera

PROVIDER: GSE31662 | GEO | 2011/08/26

SECONDARY ACCESSION(S): PRJNA145395

REPOSITORIES: GEO

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Grape skin transcriptome in the berries cultured in vitro treated with exogenous abscisic acid

Project description:The softened green berries of Cabernet Sauvignon were cultured in a Petri dish. 0.3M sucrose solution with (±)-abscisic acid (ABA) and without (±)-ABA (control) were applied to the Petri dishes. The berry skins were sampled at 3 and 10 days after the application. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV17 at PLEXdb. ABA treated - time: 3 days(2-replications); ABA treated - time: 10 days(2-replications); Non-treated (control) - time: 3 days(2-replications); Non-treated (control) - time: 10 days(2-replications)

2011-08-25 | E-GEOD-31662 | biostudies-arrayexpress

Grape skin transcriptome in the berries grown on the vine treated with exogenous abscisic acid

2011-08-27 | GSE31664 | GEO

Grape skin transcriptome in the berries grown on the vine treated with exogenous abscisic acid

Project description:The experiment was conducted using grapevines of Cabernet Sauvignon grown in a field. At veraison, the clusters in the vines were sprayed with a 400 mgl-1(+)-abscisic acid (ABA) solution. The ABA treated berries and non-treated berries (control) were harvested at 14 and 28 days after veraison and peeled. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV16 at PLEXdb. ABA treated - developmental stage name: 14 days after veraison(3-replications); ABA treated - developmental stage name: 28 days after veraison(3-replications); untreated (control) - developmental stage name: 14 days after veraison(3-replications); untreated (control) - developmental stage name: 28 days after veraison(3-replications)

2011-08-26 | E-GEOD-31664 | biostudies-arrayexpress

High temperature effect on Cabernet Sauvignon berries

2011-08-26 | GSE31675 | GEO

High temperature effect on Cabernet Sauvignon berries

Project description:The experiment was conducted using four 11-year-old potted grapevines of V. vinifera L. cv. Cabernet Sauvignon grafted on SO4 (Selection Oppenheim No. 4) grown in a phytotron. Vines were trained on a Guyot trellising system and each vine carried 4-10 clusters of grapes. The experiment started approximately 1 week before veraison, when berry softening started, and continued to fruit maturity. The two temperature regimes consisted of a high day (06.00–20.00 h) temperature (max.35C) and a control (max. 25C). Under both conditions, the night-time (20.00–06.00 h) temperature was 20C. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kentaro Mori. The equivalent experiment is VV9 at PLEXdb developmental stage: 2 weeks - temperature: High (2-replications); developmental stage: 2 weeks - temperature: Control(2-replications); developmental stage: 4 weeks - temperature: High (2-replications); developmental stage: 4 weeks - temperature: Control(2-replications); developmental stage: 6 weeks - temperature: High (2-replications); developmental stage: 6 weeks - temperature: Control(2-replications)

2011-08-25 | E-GEOD-31675 | biostudies-arrayexpress

short term abiotic stress Cabernet Sauvignon

Project description:Cabernet Sauvignon plants grown in a hydroponic drip system were treat with 120 mM salt (10:1 NaCl:CaCl), Polyethylene Glycol, cold (5 C) or unstressed. Shoots with leaves were collected at 0, 1, 4, and 8 hours for all treatments, and at 24 hours for all except cold. ****[PLEXdb (http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Elizabeth Tattersall. The equivalent experiment is VV1 at PLEXdb.]

2011-08-23 | GSE31594 | GEO

Long-term Salt & Water Stress in Grapes

Project description:Potted Cabernet Sauvignon vines in the greenhouse were exposed to irrigated controls, non-irrigated water-deficits, and saline treatments for 16 days. Plant shoot tips were harvested every 4 days (0,4,8,12, and 16 days) to measure the progression of changes of global gene expression due to the stress. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Grant Cramer. The equivalent experiment is VV2 at PLEXdb.

2011-08-26 | GSE31677 | GEO

Gene expression associated with compatible viral diseases in berry

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2011-08-26 | GSE31660 | GEO

Integrated omics analysis reveals the norisoprenoid responses in Vitis vinifera L. cv. Cabernet Sauvignon berries to abscisic acid and synthetic auxin

Project description:The abscisic acid (ABA) increase and auxin decline are both indicators of ripening initiation in grape berry, and norisoprenoid accumulation also start at around the onset of ripening. To investigate the transcriptional and posttranscriptional regulation of the ABA and synthetic auxin 1-naphthaleneacetic acid (NAA) on norisoprenoid production, we performed time series GC-MS and RNA-seq analyses on Cabernet Sauvignon grape berries from pre-veraison to ripening. Higher levels of free norisoprenoids were found in ABA-treated mature berries in two consecutive seasons, and both free and total norisoprenoids were significantly increased by NAA in one season. The expression pattern of known norisoprenoid-associated genes in all samples and the up-regulation of specific alternative splicing isoforms of VviDXS and VviCRTISO in NAA-treated berries were predicted to contribute to it. Combined weighted gene co-expression network analysis (WGCNA) and promoter motif prediction analysis suggested that GATA26 and GATA28 could be potential regulators of norisoprenoid accumulation. Finally, the network analysis uncovered the interaction between previously identified switch genes, hormone-related genes and norisoprenoid-associated genes.

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Gene expression associated with compatible viral diseases in berry

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