Project description:miRNA profiling of mouse kidney cortex comparing control vs. low sodium diet + captopril treatment to induce renin expression. Two condition experiment: control vs treated; biological replicates: individual mice - 3 control, 3 treated. One replicate per array.
Project description:Attempt to identify genes whose expression changed in the kidney cortex with angiotensin converting enzyme inhibition. Eleven week-old male C57BL6 mice were treated with captopril at 10mg/kg/day in drinking water for 7 days. The kidney cortex was surgically excised and total RNA was isolated using Trizol (Invitrogen) from three treated and three control mice and was further purified using RNeasy MinElute Cleanup spin columns (Qiagen) according to manufacturer’s instructions. Probes generated from the resulting RNA were hybridized to Illumina Expression BeadChips (mouseWG-6_V2).
Project description:Quantification of acetylation stoichiometry from mitochondrial enriched proteins from mouse livers. Conditions compared include genotype, diet, and age; i.e. Sirt3 KO vs Sirt3 WT, caloric restricted (CR) diet vs control diet (CD), and 5 months old vs 25 months old.
