Project description:A comparison of mRNA profiles of insecticide resistant and susceptible genotypes. Compare two biological replicates from resistant and susceptible genotypes
Project description:Comparison of insecticide resistant mosquitoes (An. arabiensis) with control samples, comparison of resistant samples with susceptible (Dongola) strain and comparison of resistant (Sennar) strain with susceptible (Dongola) strain
Project description:Comparison of an adult female pyrethroid insecticide resistant population of Anopheles gambiae ss, from Tiefora Burkina Faso, to a lab susceptible An. gambiae ss Kisumu sampled in 2013.
Project description:This microarray study aimed at comparing constitutive gene expression levels between an Aedes aegypti insecticide-resistant strain (Imida-R) selected at the larval stage with the neonicotinoid insecticide imidacloprid for 10 generations and the parental strain (Bora-Bora) susceptible to all insecticides. Strains comparison was performed at both larval (4th stage larvae) and adult (3 days-old adults females, non-blood fed) stages.
Project description:Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. The coding trimmed sequences from Augustus were then used as the mapping reference for the individual FASTQ files from each strain. trace reads were mapped using RSEM to generate FPKM estimates. Gene expression levels were compared to the ALHF strain using EdgeR to identify genes that were downregulated in both aabys and CS. RNA Seq profiling of the mRNA of adult Musca domestica with different autosomal contributions from insecticide resistant and susceptible strains (whole insect pooled samples)
Project description:The aim of this experiment was to compare the transciptome of the peach-potato aphid (Myzus persicae) clone 4106a (a laboratory insecticide-susceptible standard collected from potato in Scotland in 2000) with clone 5191A (an insecticide resistant aphid clone collected from tobacco in Greece in 2007) to identify which genes are over or underexpressed in the resistant phenotype.
Project description:A microarray experimental design with dye balancing was adopted to compare the gene expression profiles of the following experimental groups; Field collected Makkah and Jeddah strains unexposed to any insecticide Fully insecticide susceptible New Orleans and Rockefeller laboratory strains. Laboratory Jeddah strain (F5) without exposure to insecticides Laboratory Jeddah strains selected for deltamethrin resistance (F5). For each comparison (field, lab selected or unselected vs susceptible), three biological replicates were used. The two susceptible strains, New Orleans and Rockefeller served as reference.
Project description:Transcripcional responses in three genotypes of Muzus persicae, each exhibiting different resistance mechanisms, in response to an anti.cholinesterase insecticide Two-condition experiment in threee different genotypes: Insecticide vs. acetone plus water. Genotypes S (exhibiting no resistance mechanism) Biological replicates: 3. Genotypes RS (resistant through a kdr sodium channel mutation) Biological replicates: 2. Genotypes MR (carrying kdr and MACE mutations) Biological replicates: 2.
Project description:Transcriptome profiling of insecticide resistant field populations of Anopheles funestus from Benin compared to a susceptible lab strain FANG
Project description:The 791spin is the spinosad-selected strain derived from 791a, a laboratory strain derived from a multi-resistant field-collected sample of houseflies. The 791a strain proved highly resistant to pyrethroids and some anticholinesterases and showed some resistance to the chitin synthesis-disrupting larvicides.In order to understand the evolution of insecticide resistance, de novo assembly of a spinosad resistant housefly strain 791spin using 454 technology was initiated Transcriptome analysis of insecticide resistant housefly strain compared to susceptible strain