Cryptococcal H99 cells grown in 8 conditions for capsule induction
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ABSTRACT: To understand the relationship between gene expression and capsule formation, H99 cells were cultured overnight at 37ºC in the following eight conditions: low iron medium with or without both 500 mM ethylenediaminetetraacetic acid (EDTA) and 10 mM bathophenanthroline disulfonate (BPDS); phosphate-buffered saline (PBS) with or without 10% v/v fetal bovine serum; Dulbecco's Modified Eagle's Medium (from Sigma) in ambient air or 5% CO2; and Littman's medium with either 0.01 µg/ml or 1 µg/ml thiamine.
ORGANISM(S): Cryptococcus neoformans var. neoformans Cryptococcus neoformans
PROVIDER: GSE31911 | GEO | 2012/01/01
SECONDARY ACCESSION(S): PRJNA155301
REPOSITORIES: GEO
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