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H99 derived Uniconazole (1ug/mL) adaptors

ABSTRACT: H99 derived Uniconazole (1ug/mL) adaptors

PROVIDER: PRJEB75213 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12945409_1.fastq.gz	Fastqsanger.gz
	ERR12945409_2.fastq.gz	Fastqsanger.gz
	ERR12945410_1.fastq.gz	Fastqsanger.gz
	ERR12945410_2.fastq.gz	Fastqsanger.gz
	ERR12945411_1.fastq.gz	Fastqsanger.gz

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1 - 5 of 54

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Project description:H99 derived 2 ug/mL uniconazole adaptors: Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 2 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen. 21 adaptors were resistant and were sequenced. H99 derived 0.25 ug/mL uniconazole adaptors: Cells of H99 were adjusted to 2500 cells/mL. The culture was supplemented with 0.25 ug/mL uniconazole. After 72 h incubation, the culture was washed, diluted and approximately 200 cells were spread on YPD-agar plate. Randomly 95 colonies were tested for resistance to uniconazole. One colony (TJ3832) was resistant and was sequenced.

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