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Effect of CovR polymorphisms on GAS gene expression

ABSTRACT: In this study we sought to determine the effects of single amino acid replacements in the control of virulence regulatory (CovR) regulatory protein on group A streptococcal global gene expression.

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE32106 | GEO | 2011/11/01

SECONDARY ACCESSION(S): PRJNA147469

REPOSITORIES: GEO

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Effect of CovR polymorphisms on GAS gene expression

Project description:In this study we sought to determine the effects of single amino acid replacements in the control of virulence regulatory (CovR) regulatory protein on group A streptococcal global gene expression. We compared the transcriptomes of the serotype M3 strain MGAS10870 with four derivative strains: 1) a strain in which CovR had been completely inactivated (CovR knockout); 2) a strain in which the Arg 144 amino acid was changed to a cysteine (CovR-R144C); 3) a strain in which the Arg 158 amino acid was changed to a cysteine (CovR-R158C); and 4) a strain in which the the Asn 193 amino acid was changed to an isoleucine (CovR-N193I). Duplicate samples of each strain were grown to mid-exponential phase and the relative transcriptomes were compared using an Affymetrix GeneChipl.

2011-11-01 | E-GEOD-32106 | biostudies-arrayexpress

CovS Inactivation Reduces CovR Promoter Binding at Diverse Virulence Factor Encoding Genes in Group A Streptococcus

Project description:We performed CovR ChIP-seq analysis in the emm1 strain MGAS2221 and its CovS kinase deficient derivative strain 2221-CovS-E281A. We identified that CovR bound in the promoter regions of nearly all virulence factor encoding genes in the CovR regulon. Additionally, direct CovR binding was observed for numerous genes encoding proteins involved in amino acid metabolism, but we found limited direct CovR binding to genes encoding other transcriptional regulators. The consensus sequence AATRANAAAARVABTAAA was present in the promoters of genes directly regulated by CovR, and mutations of highly conserved positions within this motif relieved CovR repression of the hasA and M2221_0187 promoters. Analysis of strain 2221-CovS-E281A revealed that binding of CovR at repressed, but not activated, promoters is highly dependent on CovR~P state. CovR repressed virulence factor encoding genes could be grouped dependent on how variation in CovR~P differentially impacted DNA binding and gene transcript levels.

2022-01-03 | GSE192795 | GEO

GAS M6 serotype, wild-type vs covR mutant

Project description:JRS4 is our wild-type M6 serotype strain and JRS948 is the isogenic covR strain. Keywords: 2-channel

2005-12-19 | GSE3367 | GEO

Evaluation of covS polymorphisms on group A streptococcal global gene expression

2015-01-24 | GSE65226 | GEO

ChIP-seq of the response regulator CovR in Group B Streptococcus (GBS)

Project description:We report the characterization of the major regulator of virulence gene expression (CovR) in Group B Streptococcus. The ChIP-seq experiments define the binding of CovR on the chromosome of the BM110 strain, a representative of the hypervirulent GBS lineage responsible of neonatal meningitis. Regulatory evolution of CovR signaling was investigated by comparing ChIP-seq done in parallel in a second GBS clinical isolate (NEM316) not belonging to the hypervirulent lineage.

2021-08-23 | GSE158046 | GEO

GAS M6 serotype, wild-type vs covR mutant

Project description:JRS4 is our wild-type M6 serotype strain and JRS948 is the isogenic covR strain. Keywords: 2-channel Four separate RNA preparations from each strain (RNA #A-D) were hybridized to duplicate arrays. Reference RNA consisted of pooled RNA from each preparation, labelled with Cy5.

2005-12-19 | E-GEOD-3367 | biostudies-arrayexpress

Evaluation of covS polymorphisms on group A streptococcal global gene expression

Project description:We sought to determine how single amino acid changes in the active site of CovS autophosphorylation and subsequent phosphorylation of the response regulator CovR affect the gene expression profile of group A streptococcus There were 6 strains analyzed, each in quadruplicate replicates: 1) wild-type GAS; 2) covS deletion strain; 3) covS with alanine at AA 280; 4) covS with valine at AA 281; 5) covS with valine at AA 284; 6) covS with alanine at AA 457

2015-01-24 | E-GEOD-65226 | biostudies-arrayexpress

Evaluating the effect of CovR phosphorylation on group A streptococcus global gene expression

Project description:We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus

2018-10-19 | GSE121313 | GEO

Gene expression-based global role of CovR in GBS A909

Project description:Global regulatory roles of the TCS response regulator CovR in GBS serotype 1a A909 Keywords: Strain comparison; global regulation

2007-10-18 | GSE9370 | GEO

Gene expression-based global role of CovR in GBS A909

Project description:Global regulatory roles of the TCS response regulator CovR in GBS serotype 1a A909 Keywords: Strain comparison; global regulation Deletion mutant was examined for alterations in gene expression

2007-10-18 | E-GEOD-9370 | biostudies-arrayexpress

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