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Transcriptional profile of pathogenic and non-pathogenic in vitro generated mycobacterial cell wall deficient forms

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mycobacterium avium subsp. paratuberculosis K-10 Mycolicibacterium smegmatis MC2 155 Mycobacterium tuberculosis H37Rv Mycobacterium avium subsp. paratuberculosis

PROVIDER: GSE32237 | GEO | 2012/11/29

SECONDARY ACCESSION(S): PRJNA147197

REPOSITORIES: GEO

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Transcriptional profile of pathogenic and non-pathogenic in vitro generated mycobacterial cell wall deficient forms

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-11-29 | E-GEOD-32237 | biostudies-arrayexpress

Differentially regulated genes induced by in vitro generated Mycobacterium tuberculosis cell wall deficient forms

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Differentially regulated genes induced by in vitro generated Mycobacterium tuberculosis cell wall deficient forms

Project description:Transcriptional profile of chemically generated Mycobacterium tuberculosis cell wall deficient forms, comparing untreated control cells (bacillary forms) and cell wall deficient cells. Two-condition experiment, MTB-K vs. MTB-Sph cells. Biological replicates: 3 controls, 3 spheroplasts, independently grown and harvested. In controls and spheroplasts, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, five replicates per array.

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Differentially regulated genes induced by in vitro generated Mycobacterium avium subspecies paratuberculosis cell wall deficient forms

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Differentially regulated genes induced by in vitro generated Mycobacterium avium subspecies paratuberculosis cell wall deficient forms

Project description:Transcriptional profile of chemically generated Mycobacterium avium subspecies paratuberculosis cell wall deficient forms, comparing untreated control cells (bacillary forms) and cell wall deficient cells. Two-condition experiment, MAP-K vs. MAP-Sph cells. Biological replicates: 3 controls, 3 spheroplasts, independently grown and harvested. In controls and spheroplasts, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, four replicates per array.

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Cell-wall deficient L. monocytogenes L-forms feature abrogated pathogenicity.

Project description:Stable L-forms are cell wall-deficient bacteria which are able to multiply and propagate indefinitely, despite the absence of a rigid peptidoglycan cell wall. We investigated whether L-forms of the intracellular pathogen L. monocytogenes possibly retain pathogenicity, and if they could trigger an innate immune response. While phagocytosis of L. monocytogenes L-forms by non-activated macrophages sometimes resulted in an unexpected persistence of the bacteria in the phagocytes, they were effectively eliminated by IFN-γ preactivated or bone marrow-derived macrophages (BMM). These findings were in line with the observed down-regulation of virulence factors in the cell-wall deficient L. monocytogenes. Absence of Interferon-β (IFN-β) triggering indicated inability of L-forms to escape from the phagosome into the cytosol. Moreover, abrogated cytokine response in MyD88-deficient dendritic cells (DC) challenged with L. monocytogenes L-forms suggested an exclusive TLR-dependent host response. Taken together, our data demonstrate a strong attenuation of Listeria monocytogenes L-form pathogenicity, due to diminished expression of virulence factors and innate immunity recognition, eventually resulting in elimination of L-form bacteria from phagocytes.

| S-EPMC4033035 | biostudies-literature

Differentially regulated genes induced by in vitro generated Mycobacterium smegmatis cell wall deficient form

2012-11-29 | GSE32133 | GEO

Differentially regulated genes induced by in vitro generated Mycobacterium smegmatis cell wall deficient form

Project description:Transcriptional profile of chemically generated Mycobacterium smegmatis cell wall deficient forms, comparing untreated control cells (bacillary forms) and cell wall deficient cells. Two-condition experiment, MSMEG-K vs. MSMEG-Sph cells. Biological replicates: 3 controls, 3 spheroplasts, independently grown and harvested. In controls and spheroplasts, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, Three replicates per array.

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pH-dependent transcriptional profile changes in iron-deficient Arabidopsis roots.

Project description:BackgroundIron is an essential element for plants and abundantly present in most mineral soils. The mobility of iron is, however, dependent on the redox potential and hydrogen activity (pH) of the soil, factors that may limit its availability to plants in particular at alkaline pHs. Iron deficiency triggers pronounced changes in the transcriptional profile of plants, inducing processes that aid in the acquisition, uptake, and translocation of iron. How ambient pH impact the transcriptional iron deficiency response has not yet been elucidated in detail.ResultsHere, we provide an RNA-seq data set that catalogs global gene expression changes of iron-deficient plants grown at either optimal (5.5) or high (7.0) pH. A suite of 857 genes changed significantly and more than twofold in expression; only 54 genes of this suite were also differentially expressed between iron-deficient and iron-sufficient plants grown at pH 5.5. Among the high pH-responsive genes, 186 were earlier shown to be responsive to short-term transfer to low pH, 91 genes of this subset were anti-directionally regulated by high and low pH. The latter subset contained genes involved in cell wall organization, auxin homeostasis, and potential hubs of yet undefined signaling circuits. Growing iron-deficient plants at high pH also modulated the transcriptional iron deficiency response observed at pH 5.5 by compromising the enzymatic reduction of ferric chelates and favoring the production of iron-mobilizing coumarins.ConclusionsIt is concluded that ambient pH is an important determinant of global gene expression which tunes iron acquisition to the prevailing edaphic conditions.

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