Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Rybp ChIP-chip in mouse ES cells

ABSTRACT: Rybp binding in wild-type, Eed-KO, and Dnmt1-KO ES cells

ORGANISM(S): Mus musculus

PROVIDER: GSE32289 | GEO | 2012/04/04

SECONDARY ACCESSION(S): PRJNA154507

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Rybp ChIP-chip in mouse ES cells

Project description:Rybp binding in wild-type, Eed-KO, and Dnmt1-KO ES cells ChIP on chip analysis was carried out using the Mouse Promoter ChIP-on-chip Microarray Set (G4490A, Agilent-014716 and Agilent-014717, Palo Alto, Calif., USA). ESCs were subjected to ChIP assay using a Rybp antibody (Santa Cruz; H-115). Purified immunoprecipitated and input DNA was subjected to T7 RNA polymerase-based amplification as described previously (van Bakel et al., 2008). Labeling, hybridization and washing were carried out according to the Agilent mammalian ChIP-on-chip protocol (ver.9.0). Scanned images were quantified with Agilent Feature Extraction software under standard conditions.

2012-04-03 | E-GEOD-32289 | biostudies-arrayexpress

Expression regulation by RYBP in mouse embryonic stem cells

Project description:Polycomb repressive complexes (PRCs) are important chromatin regulators of ES cell function. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B, and has been suggested to participate in localizing Polycomb complexes to their targets. Moreover, constitutive inactivation of RYBP precludes ES cell formation. Here we have used ES cells conditionally deficient in RYBP to investigate RYBP function. Chromosome immunoprecipitation on a chip (ChIP-chip) of RYBP and microarray experiments were performed using wild type and knocked-out ES cells. Gene expression profiling of WT, conditionally deficient in RYBP with or without Yaf2 RNAi, and ChIP-chip of RYBP on promoters of WT, Dnmt1-KO or Eed-KO ES cells.

2012-04-03 | E-GEOD-32294 | biostudies-arrayexpress

Gene expression profile in Yaf2 KD and/or RYBP KO ES cells.

Project description:Gene expression change by Yaf2 KD in wild type or RYBP KO ES cells.

2012-04-04 | GSE32288 | GEO

Gene expression profile in Yaf2 KD and/or RYBP KO ES cells.

Project description:Gene expression change by Yaf2 KD in wild type or RYBP KO ES cells. Total RNAs were extracted from the respective ES cells, and were subjected to microarray analysis using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays

2012-04-03 | E-GEOD-32288 | biostudies-arrayexpress

Expression regulation by RYBP in mouse embryonic stem cells

Project description:Polycomb repressive complexes (PRCs) are important chromatin regulators of ES cell function. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B, and has been suggested to participate in localizing Polycomb complexes to their targets. Moreover, constitutive inactivation of RYBP precludes ES cell formation. Here we have used ES cells conditionally deficient in RYBP to investigate RYBP function. Chromosome immunoprecipitation on a chip (ChIP-chip) of RYBP and microarray experiments were performed using wild type and knocked-out ES cells. This SuperSeries is composed of the SubSeries listed below.

2012-04-04 | GSE32294 | GEO

Expression data from wild-type and Rybp-deficient ES cells

Project description:We used microarrays to investigate a global change in gene expression by conditional depletion of Rybp in mouse ES cells. Total RNAs were extracted from wild-type and Rybp-deficient ES cells, and were subjected to microarray analysis using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays

2012-04-03 | E-GEOD-32287 | biostudies-arrayexpress

Expression data from wild-type and Rybp-deficient ES cells

Project description:We used microarrays to investigate a global change in gene expression by conditional depletion of Rybp in mouse ES cells.

2012-04-04 | GSE32287 | GEO

Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity

Project description:These data include the genome wide location of different histone modifications by ChIP sequencing in mouse ES cells, and RNA Seq data generated from wild type and EED KO mouse ES cells and knocked down for unrelated protein and Setd2 protein. ChIP-Seq: Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type mouse E14 ES cells, wild type E36 ES cells, EED KO E36 ES cells, wild type Embryoid bodies (Ebs), EED KO Embryoid bodies (Ebs EED KO) using specific antibody against different histone modifications. RNA-Seq: Total RNA extracted from wild type E36 ES cells, EED KO E36 ES cells, wild type E36 Embryoid bodies (Ebs), EED KO Embryoid bodies (Ebs EED KO), E14 Ctrl KD, E14 Setd2 KD.

2013-12-05 | E-GEOD-51006 | biostudies-arrayexpress

Transcription profiling of mouse ES cells and various differentiated cell types

Project description:We used microarrays to detail the role of Polycomb proteins including Ezh2 and Eed in maintaining ES cell identity and executing pluripotency. Experiment Overall Design: To assay the global effects of the loss of polycomb proteins (Ezh2 or Eed) in embryonic stem (ES) cells , we compared the expression profiles of homologuous Ezh2 or Eed knockout ES cells to wild-type ES cells in undifferentiated or differentiated condition.

2009-01-14 | E-GEOD-12982 | biostudies-arrayexpress

Chip-Seq Raw data in LSD1 WT and KO mouse ES cells

Project description:LSD1, H3K4me1 and DNMT1 binding in LSD1 WT and KO mouse ES cells

| PRJEB78609 | ENA