Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Transcription profiling of mouse ES cells and various differentiated cell types

ABSTRACT: We used microarrays to detail the role of Polycomb proteins including Ezh2 and Eed in maintaining ES cell identity and executing pluripotency. Experiment Overall Design: To assay the global effects of the loss of polycomb proteins (Ezh2 or Eed) in embryonic stem (ES) cells , we compared the expression profiles of homologuous Ezh2 or Eed knockout ES cells to wild-type ES cells in undifferentiated or differentiated condition.

ORGANISM(S): Mus musculus

SUBMITTER: Xiaohua Shen 

PROVIDER: E-GEOD-12982 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency.

Shen Xiaohua X   Liu Yingchun Y   Hsu Yu-Jung YJ   Fujiwara Yuko Y   Kim Jonghwan J   Mao Xiaohong X   Yuan Guo-Cheng GC   Orkin Stuart H SH  

Molecular cell 20081101 4

Trimethylation on H3K27 (H3K27me3) mediated by Polycomb repressive complex 2 (PRC2) has been linked to embryonic stem cell (ESC) identity and pluripotency. EZH2, the catalytic subunit of PRC2, has been reported as the sole histone methyltransferase that methylates H3K27 and mediates transcriptional silencing. Analysis of Ezh2(-/-) ESCs suggests existence of an additional enzyme(s) catalyzing H3K27 methylation. We have identified EZH1, a homolog of EZH2 that is physically present in a noncanonica  ...[more]