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Expression profiling of wild-type and set3D fission yeast strains

ABSTRACT: In fission yeast the SET domain protein, Set3p is required for the reliable execution of cytokinesis. To address whether the deletion of the set3 gene might selectively alter expression of cytokinesis genes, expression profiling of wildtype and set3D strains was performed.

ORGANISM(S): Schizosaccharomyces pombe Saccharomyces cerevisiae

PROVIDER: GSE33228 | GEO | 2012/01/01

SECONDARY ACCESSION(S): PRJNA149163

REPOSITORIES: GEO

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Expression profiling of wild-type and set3D fission yeast strains

Project description:In fission yeast the SET domain protein, Set3p is required for the reliable execution of cytokinesis. To address whether the deletion of the set3 gene might selectively alter expression of cytokinesis genes, expression profiling of wildtype and set3D strains was performed. Wild-type or set3D strains were grown to mid-log phase in YES media and treated with 0.5 mM LatA (or the solvent control, DMSO) for three hours at 30 degrees C. Three biological replicates were performed.

2012-01-01 | E-GEOD-33228 | biostudies-arrayexpress

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Project description:Connections between the protein kinases that function within complex cell polarity networks are poorly understood. Rod-shaped fission yeast cells grow in a highly polarized manner, and genetic screens have identified many protein kinases required for polarized growth and cell shape. Kin1, the sole MARK/PAR-1 family kinase in fission yeast, regulates cell polarity and cytokinesis through unknown mechanisms. We performed a large-scale phosphoproteomics screen to identify and characterize Kin1 substrates in cells.

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