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Gene expression by Retinoic Acid in mouse Dendritic Cells

ABSTRACT: The purpose of this study was to determine and clarify the retinoic effect on the gene expression profile for mouse dendritic cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE34324 | GEO | 2013/02/26

SECONDARY ACCESSION(S): PRJNA151481

REPOSITORIES: GEO

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Gene expression by Retinoic Acid in mouse Dendritic Cells

Project description:The purpose of this study was to determine and clarify the retinoic effect on the gene expression profile for mouse dendritic cells. Dendritic cells were prepared from marrow cells by culturing them for 10 days with GM-CSF (20 ng/ml). These cells were cultured with the following reagents from the beginning of the culture: Two groups of dendritic cell conditions (Control, all-trans-retinoic acid (10 nmol) and Ro41-5253 (100 nmol)) with and without LPS(1 ug/ml). The above conditions were done in BALB/c and BL6 mouse strains and compared for reproducible gene expression.

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Project description:Observational studies in human suggest involvement of vitamin A/retinoic acid (RA) signaling in the regulation of airway smooth muscle (ASM) function, but the precise mechanisms by which RA impacts ASM phenotype is not clear. Here, we generated trascriptional profiles from two different models of RA-sufficient and RA-deficient mouse ASM in order to determine the molecular targets of RA in ASM (VAS/VAD, CTR/BMS) Mouse ASMs were isolated from Acta2-hrGFP;Cspg4-DsRED adult mice using flow cytometry. VAS mice were given normal, vitamin A-sufficient diet (15 IU vitamin/g) while the VAD mice were given 3 days of vitamin A-deficient diet (< 0.1 IU vitamin/g). CTR/BMS mouse ASMs were also isolated from Acta2-hrGFP;Cspg4-DsRED adult mice using flow cytometry. CTR mice were given 3 days of powdered, vitamin A sufficient diet mixed with vehicles (corn oil) while the BMS diet were given 3 days of powdered, vitamin A sufficient diet mixed with a potent pan-RA-receptor antagonist (BMS, 5 mcg/g).

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