Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with Haemophilus parasuis.
Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with HPS4.
Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with Haemophilus parasuis. Healthy Pigs were intratracheally challenged with H.parasuis strain 0165. And the PAMs were isolated at 6 dpi. RNA extraction were extracted from PAMs that obtained from infection pigs and control pigs and hybridization on Affymetrix microarrays. We sought to obtain the differently expressed genes that related to H.parasuis infection to understand the mechanism of PAMs against H.parasuis.
Project description:To understand the mechanism of the adaptations, global gene expression profiles of the cecropin B--resistant strains of Haemophilus parasuis, The development of cecropin B (CB) resistance in H. parasuis SH0165 by exposing SH0165 using various concentrations of CB was investigated. One colony of bacterial cultures grown on TSA containing 30μg/mL CB was named CBR30, CBR30 cultured in TSA without CB for 50 passages was named CBR30-50. we used microarray technology to analyze the variation of the H. parasuis SH0165 transcriptional profile in CBR30 and CBR30-50. Our analysis to identify several genes whose system could be involved in inorganic ion transport, amino acid transport, and metabolism. Quantitative PCR was used to validate the differential expression of selected genes.
Project description:To understand the mechanism of the adaptations, global gene expression profiles of the cecropin B--resistant strains of Haemophilus parasuis, The development of cecropin B (CB) resistance in H. parasuis SH0165 by exposing SH0165 using various concentrations of CB was investigated. One colony of bacterial cultures grown on TSA containing 30M-NM-<g/mL CB was named CBR30, CBR30 cultured in TSA without CB for 50 passages was named CBR30-50. we used microarray technology to analyze the variation of the H. parasuis SH0165 transcriptional profile in CBR30 and CBR30-50. Our analysis to identify several genes whose system could be involved in inorganic ion transport, amino acid transport, and metabolism. Quantitative PCR was used to validate the differential expression of selected genes. The H. parasuis SH0165 control group was normal culture with no tilmicosin at OD600 >0.3 (12 h). The H. parasuis CBR30 was normal culture with 30M-NM-<g/mL cecropin B at OD600 >0.3 (20 h). The H. parasuis CBR30-50 was normal culture with no cecropin B at OD600 >0.3 (12 h). Three independent experiments were performed on each group using a different sample for each experiment.
Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with HPS4. Healthy Pigs were inoculated intranasally with 2 ml of 4.5×108CFU/ml colony forming units of HPS4 strain MD0322. And the PAMs were isolated at 28 dpi. RNA extraction were extracted from PAMs that obtained from infection pigs and control pigs and hybridization on Affymetrix microarrays.
Project description:Purpose: Identify differentially expressed genes in pig lung, compared to growth on chocolate agar plate. Methods: In order to study the gene expression at this location, we sequenced ex vivo and in vivo H. parasuis transcriptome using a metatranscriptomic approach. Gene expression was compared with conventional plate culture. Results: down-regulation of anabolic and catabolic pathways, coupled with up-regulation of membrane-related genes involved in carbon acquisition, iron binding and pathogenesis. Conclusions: This data sheds some light on the scarcely studied in vivo transcriptome of H. parasuis, revealing nutritional virulence as an adaptive strategy for host survival.
