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Comparing expression profiles of NIH3T3 cells with and without LPAR1 mutation

ABSTRACT: To explore the signaling pathways of mutant LPAR1, we performed gene expression profiling for NIH3T3 cells expressing wild-type and mutant LPAR1 receptors using Affymetrix mouse 430 2.0 arrays

ORGANISM(S): Mus musculus

PROVIDER: GSE34629 | GEO | 2012/12/22

SECONDARY ACCESSION(S): PRJNA151279

REPOSITORIES: GEO

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Comparing expression profiles of NIH3T3 cells with and without LPAR1 mutation

Project description:To explore the signaling pathways of mutant LPAR1, we performed gene expression profiling for NIH3T3 cells expressing wild-type and mutant LPAR1 receptors using Affymetrix mouse 430 2.0 arrays 6 RNA samples from the same cell line. 3 samples contained LPAR1 mutant and 3 samples were wild-type. Cells were harvested at 0, 60, and 120 minutes.

2012-12-22 | E-GEOD-34629 | biostudies-arrayexpress

Transcription profiling of mouse cells sensitive to necrosis (L929) and cells sensitive to apoptosis (NIH3T3).

Project description:To explore gene expression profiles of cells sensitive to necrosis (such as L929 cells) and those sensitive to apoptosis (such as NIH3T3 cells), we conducted expression microarray analysis of L929 cells and NIH3T3 cells. Experiment Overall Design: Total RNA of 2 samples of unstimulated L929 cells or 2 samples of unstimulated NIH3T3 cells were extracted by Trizol followed by RNeasy. The quantity, purity and integrity of RNA were evaluated by UV spectrophotometry and RNA-nano Bioanalyzer. Sample processing and hybridization on Mouse Genome 430 2.0 GeneChip microarrays (Affymetrix) were performed according to manufacturerâs instructions. Genes that are differentially expressed in L929 cells relative to NIH3T3 cells were identified as having an FDR-adjusted p-value <0.05 and a fold-change >1.5.

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Phactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts

Project description:It was previously shown that expression of an activated Phactr1 mutant (Phactr1XXX) that constitutively forms the Phactr1/PP1 phosphatase holoenzyme, induces F-actin rearrangements in NIH3T3 fibroblasts. Expression of the Phactr1 PP1-binding domain (C-terminal) alone is also sufficient to induce such cytoskeletal changes. In contrast, expression of Phactr1XXXC derivative, which lacks the PP1 binding sequences does not result in alteration of cytoskeletal morphology (Wiezlak et al., 2012). These observations suggest that Phactr1/PP1 dephosphorylates target proteins involved in cytoskeletal dynamics. To identify potential Phactr1/PP1 substrates, we used differential SILAC phosphoproteomics in NIH3T3 cells inducibly expressing Phactr1XXX, Phactr1XXXC constructs or vector alone. Over 3000 phosphorylation sites were quantified, among which we determined Phactr1/PP1 target dephosphorylation sites by comparative analysis.

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Nutrient-dependent growth of NIH3T3 and NIH3T3 K-ras cell lines.

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Murine NIH3T3 Cells: Wild vs. BRAFV600E Transfected

Project description:Transcriptional profiling of mouse NIH3T3 cells comparing control NIH3T3 cells transfected with a pEFm6-BRAF with cells transfected with pEFm6-BRAFV600E. Goal was to determine the effects of BRAFV600E gene transfection on global mouse NIH3T3 cells gene expression.

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Project description:Expression profiling of sCA-siRNA delivery in NIH3T3 cells (siControl vs. siTIMP1) Goal was to determine the off target effects of sCA-siTIMP1 in NIH3T3 cells.

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Transcription profiling of oncovav- and oncovav2-transformed NIH3T3 cells vs parental NIH3T3 controls

Project description:Effect of the overexpression of the oncogenic forms of the Vav2 and Vav proteins in the NIH3T3 cell line. oncovav- and oncovav2-transformed NIH3T3 cells are compared to the parental NIH3T3 controls under exponential growth conditions

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Nutrient-dependent growth of NIH3T3 and NIH3T3 K-ras cell lines.

Project description:Expression profiling of normal NIH3T3 and transformed NIH3T3 K-ras cell lines grown for 72 hours in optimal glucose availability (25 mM glucose) or low glucose availability (1 mM). Low glucose induces apoptosis in transformed cells as compared to normal ones. We performed genome-wide analysis by using Affymetrix GeneChip oligonucleotide microarrays to identify those genes whose expression levels are modulated by glucose availability in NIH3T3 and NIH3T3 K-ras cell lines. The cells, to be used for the transcription analysis, were collected at 18h from the initial seeding, time corresponding to the change of medium (25 or 1 mM glucose, indicated as T0) and then at 24, 48 and 72h upon medium change.

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Expression data comparing control (day 0) and active (day 3) liver regeneration in mice that have undergone partial hepatectomy

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