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Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve

ABSTRACT: We provide ChIP-Seq analysis of Egr2 and Sox10 transcription factor binding in Schwann cells of rat peripheral nerve

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE35132 | GEO | 2012/04/01

SECONDARY ACCESSION(S): PRJNA150811

REPOSITORIES: GEO

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Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve

Project description:We provide ChIP-Seq analysis of Egr2 and Sox10 transcription factor binding in Schwann cells of rat peripheral nerve ChIP-Seq analysis of Egr2 and Sox10 binding in P15 rat sciatic nerve. Wiggle files of negative log of posterior probability determined by Mosaics.

2012-04-01 | E-GEOD-35132 | biostudies-arrayexpress

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Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve.

Project description:Myelin is essential for the rapidity of saltatory nerve conduction, and also provides trophic support for axons to prevent axonal degeneration. Two critical determinants of myelination are SOX10 and EGR2/KROX20. SOX10 is required for specification of Schwann cells from neural crest, and is required at every stage of Schwann cell development. Egr2/Krox20 expression is activated by axonal signals in myelinating Schwann cells, and is required for cell cycle arrest and myelin formation. To elucidate the integrated function of these two transcription factors during peripheral nerve myelination, we performed in vivo ChIP-Seq analysis of myelinating peripheral nerve. Integration of these binding data with loss-of-function array data identified a range of genes regulated by these factors. In addition, although SOX10 itself regulates Egr2/Krox20 expression, leading to coordinate activation of several major myelin genes by the two factors, there is a large subset of genes that are activated independent of EGR2. Finally, the results identify a set of SOX10-dependent genes that are expressed in early Schwann cell development, but become subsequently repressed by EGR2/KROX20.

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Sox10 Binding Analysis in the Central and Peripheral Nervous System

Project description:ChIP-seq of Sox10 in spinal cord and sciatic nerve was performed to determine shared and unique binding sites for Sox10 in oligodendrocytes and Schwann cells, respectively. Sox10 is required for both Schwann cell and oligodendrocyte development. In addition, differentiation of myelinating glia is dependent upon axonal signaling, so these studies were performed in vivo.

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Histone H3K4 trimethylation in rat peripheral nerve

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H3K27me3 ChIP-seq in rat peripheral nerve

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