Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of Francisella tularensis genes affected by iron limitation

ABSTRACT: Three pairs of total RNA preparations from F. t. LVS grown under iron-replete and iron-restricted conditions were used in the DNA microarray. Seven arrays were used to examine the genes affected by iron limitation. Keywords: Dual Channel (Cy5/Cy3) arrays

ORGANISM(S): Francisella tularensis

PROVIDER: GSE3622 | GEO | 2006/07/26

SECONDARY ACCESSION(S): PRJNA93725

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Identification of Francisella tularensis genes affected by iron limitation.

Project description:Cells of an attenuated live vaccine strain (LVS) of F. tularensis grown under iron-restricted conditions were found to contain increased quantities of several proteins relative to cells of this same strain grown under iron-replete conditions. Mass spectrometric analysis identified two of these proteins as IglC and PdpB, both of which are encoded by genes located in a previously identified pathogenicity island in F. tularensis LVS. Regions with homology to the consensus Fur box sequence were located immediately in front of the iglC and pdpB open reading frames (ORFs), and in silico analysis of the F. tularensis Schu4 genome detected a number of predicted 5' untranslated regions that contained putative Fur boxes. The putative Fur box preceding Francisella iron-regulated gene A (figA) had the highest degree of identity with the consensus Fur box sequence. DNA microarray analysis showed that nearly 80 of the genes in the F. tularensis LVS genome were up- or down-regulated at least twofold under iron-restricted growth conditions. When tested for possible siderophore production by means of the Chrome Azurol S assay, a wild-type F. novicida strain produced a large reaction zone whereas its figA mutant produced very little reactivity in this assay. In addition, a cross-feeding experiment demonstrated that this siderophore-like activity produced by the wild-type F. novicida strain could enhance the ability of the F. novicida figA mutant to grow under iron-restricted conditions. This study provides the first identification of iron-regulated genes in F. tularensis LVS and evidence for the production of a siderophore-like molecule by F. novicida.

| S-EPMC1489736 | biostudies-literature

Francisella tularensis

Project description:LVS iclR deletion mutant vs. wild-type LVS

| PRJNA130979 | ENA

Gene expression profiling in Nlrp3 deficient mice infected with Francisella tularensis

Project description:This study investigated gene expression profile in wild type and nlrp3 deficient mice infected with F. tularensis LVS. RNA-sequencing was performed to determine the transcript levels of genes using total RNA extracted from lungs of wild type C57BL/6 and Nlrp3-/- mice infected with 1 × 104 CFU of F. tularensis LVS on days 3 and 7 post-infection.

2021-10-13 | GSE183003 | GEO

Comprehensive transposon insertion sequencing screen in Francisella tularensis LVS

Project description:We used transposon insertion sequencing (Tn-Seq) to identify the genes that are required for in vitro growth and intramacrophage growth of the live vaccine strain of F. tularensis (LVS).

2020-07-15 | GSE138658 | GEO

Transcriptomic responses to a live-attenuated Francisella tularensis vaccine

Project description:Impact of live attenuated F. tularensis vaccine (DVC-LVS) on PBMC poly(A)-RNA expresssion in 10 subjects over time (Days 1, 2 ,7, and 14 post-vaccination) relative to pre-vaccination (Day 0).

2020-06-30 | GSE149809 | GEO

Novel Small RNA Modulates Virulence in Francisella tularensis

Project description:Most sRNAs control the expression of target genes by interacting with their mRNA. The fvr1 (Francisella virulence RNA 1) gene is found in the IGR between FTL_0777 and FTL_0778 and its sequence does not overlap those of the flanking genes, indicating that its target(s) is located in trans. Base-pairing between a sRNA and target mRNA generally leads to changed translation and often the stability of the mRNA is affected as well. Therefore, to identify possible targets of Fvr1, we compared the transcriptomes of the LVS?fvr1(LVS, for Live Vaccine Strain) and LVS/pfvr1+ strains after growth in liquid broth.

2012-01-31 | E-MEXP-3424 | biostudies-arrayexpress

Transcription profiling of wild type and relA spoT mutant Francisella tularensis

Project description:Global gene expression profile in an LVS relA spoT mutant compared to wild-type LVS

2010-01-01 | E-MEXP-1818 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis to spermine

Project description:Francisella tularensis LVS was grown in MH broth in the presence or absence of 200uM spermine. RNA was harvasted from overnight (16 hour) cultures and processed for microarray hybridization.

2009-11-20 | E-MEXP-2327 | biostudies-arrayexpress

Francisella tularensis

Project description:qRT-PCR on Francisella tularensis LVS and ShuS4 strain during macrophage infection

| PRJNA140967 | ENA

Transcription profiling of Francisella tularensis LVS after heat shock

Project description:Effect of temperature upshift at 44°C for 30 minutes on transcription in Francisella tularensis LVS

2009-07-01 | E-MEXP-2037 | biostudies-arrayexpress