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Murine primary dendritic cells: Control vs. Influenza-infected vs. polyI:C stimulated.

ABSTRACT: MIcroRNA expression profiling of primary murine splenic dendritic cells (Flt3L expanded) comparing untreated cells to cells infected with Influenza A or stimulated with polyI:C in vitro.

ORGANISM(S): Rattus norvegicus Mus musculus Human gammaherpesvirus 8 Human alphaherpesvirus 1 JC polyomavirus Betapolyomavirus macacae Murid gammaherpesvirus 4 Human immunodeficiency virus 1 Homo sapiens Human betaherpesvirus 5 Betapolyomavirus hominis human gammaherpesvirus 4 Murid betaherpesvirus 1

PROVIDER: GSE36316 | GEO | 2012/12/05

SECONDARY ACCESSION(S): PRJNA153357

REPOSITORIES: GEO

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Murine primary dendritic cells: Control vs. Influenza-infected vs. polyI:C stimulated.

Project description:MIcroRNA expression profiling of primary murine splenic dendritic cells (Flt3L expanded) comparing untreated cells to cells infected with Influenza A or stimulated with polyI:C in vitro. Three-condition experiment, Influenza A/PR/8/34 virus MOI=10 vs polyI:C 30 ug/mL vs. mock treated; RNA collected after 8 hours. 3 Independent biological replicates from cells prepared on different days. One replicate per array.

2012-12-05 | E-GEOD-36316 | biostudies-arrayexpress

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Splenic CD8+ and CD8- DCs

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Project description:PolyI:C stimulation of KO vs. WT BMDMs

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Project description:Pancreas tissue derived from mice with intraperitoneal injection of saline, cerulein or polyI:C plus cerulein, total RNA was extracted and deep sequenced to compare the gene expression profiles among control, acute pancreatitis and intervention of polyI:C on acute pancreatitis.

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Gene expression changes in the conceptus following maternal exposure to PolyI:C

Project description:We report the global transcript changes in the rat conceptus following maternal exposure to PolyI:C

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Expression data from monocyte-derived macrophages after stimulation with mock, LPS, PolyI:C and P3C.

Project description:Pre-stimulation of MDMs with LPS (signals via MyD88 and TRIF dependent pathways) and PolyI:C (signals via a TRIF dependent pathway) leads to a reduced viral infection. In contrast, pre-stimulation with P3C (signals via MyD88 dependent pathway) does not lead to a reduced viral infection. This microarray was performed to find genes that are specifically upregulated in LPS and PolyI:C stimulated MDMs but not P3C stimulated MDMs. So to give us leads into the mechanism involved in the reduction of viral infection. MDMs of four different donors were stimulated for 4h with mock, LPS, PolyI:C or P3C. RNA was isolated and gene expression of these cells was assessed. Gene expression of LPS, PolyI:C and P3C stimulated MDMs was compared to mock.

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Gene expression comparison among spleen dendritic cells, brain microglia, brain dendritic cells and bone marrow monocytes

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Expression data from monocyte-derived macrophages after stimulation with mock, LPS, PolyI:C and P3C.

2013-07-01 | GSE41295 | GEO

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