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Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

ABSTRACT: A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures disp[lay a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods

ORGANISM(S): Homo sapiens

PROVIDER: GSE36931 | GEO | 2012/10/01

SECONDARY ACCESSION(S): PRJNA157115

REPOSITORIES: GEO

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Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

Project description:A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures disp[lay a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods 1 colour; Cy3 labelled, 5 biological replicates for each culture condition, A549s grown in RWVs (RWV) compared to A549 grown in monolayers (mono)

2012-10-01 | E-GEOD-36931 | biostudies-arrayexpress

Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s infected with F. tularensis SchuS4 over a 22 hour time-course (2, 6 and 22 h post infection)

2012-12-01 | GSE40808 | GEO

Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s infected with F. tularensis SchuS4 over a 22 hour time-course (2, 6 and 22 h post infection)

Project description:A549 epithelial cells grown under two different conditions were compared. A549s grown in rotating wall vessel cultures display a more in-vivo like phenotype. The data here describe the transcriptional differences between the two culture methods when infected with Francisella tularensis SchuS4 over a 22 hour time-course 1 colour; Cy3 labelled, 6 biological replicates for each culture condition and time-point, A549s grown in RWVs (RWV) compared to A549 grown in monolayers (mono), Infected vs. NaM-CM-/ve

2012-12-01 | E-GEOD-40808 | biostudies-arrayexpress

Homo sapiens

Project description:Rotating wall vessel (RWV) grown A549s compared to monolayer grown A549s

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Gene expression and survival changes in Saccharomyces cerevisiae during suspension culture.

Project description:This study explores the connection between changes in gene expression and the genes that determine strain survival during suspension culture, using the model eukaryotic organism, Saccharomyces cerevisiae. The Saccharomyces cerevisiae homozygous diploid deletion pool, and the BY4743 parental strain were grown for 18 hours in a rotating wall vessel, a suspension culture device optimized to minimize the delivered shear. In addition to the reduced shear conditions, the rotating wall vessels were also placed in a static position or in a shaker in order to change the amount of shear stress on the cells. Keywords: shear stress, time course

2006-06-15 | GSE2818 | GEO

3D culture of AIDS-NHL cells influences gene expression related to B-cell development, proliferation and survival

Project description:Full title: Three-dimensional culture of AIDS-NHL cells influences gene expression related to B-cell development, proliferation and survival The AIDS-NHL-derived cell line, UMCL01-101, representing diffuse large B-cell lymphoma of immunoblastic morphology (AIDS-IBL), was grown in conventional, static suspension culture or three-dimensionally (3D) in the Rotating Wall Vessel (RWV) bioreactor. The objective was to assess the impact on gene expression of growth as a three-dimensional tissue assembly. Global gene expression analysis was performed on UMCL01-101 cells grown under either condition using Affymetrix microarray. UMCL01-101 cells were cultured in the Rotating Wall Vessel bioreactor to form 3D assemblies, or in conventional suspension culture, for 15 days. RNA was prepared from triplicate samples under each growth condition and submitted for microarray analysis.

2010-03-07 | E-GEOD-18826 | biostudies-arrayexpress

3D culture of AIDS-NHL cells influences gene expression related to B-cell development, proliferation and survival

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Vessel Forming Mesenchymal Stromal Cell Populations

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Single cell transcriptomics profiling of vessel co-option

Project description:Vessel co-option is an alternative mode of tumor vascularization, which contributes to resistance to anti-angiogenic therapy (AAT). In contrast to vessel sprouting (angiogenesis), knowledge about the mechanisms underlying vessel co-option is minimal, precluding therapeutic strategies. We therefore single-cell RNA-sequenced 31,964 cells from a murine lung metastasis model with vessel co-option, characterized by resistance to AAT. Unexpectedly, co-opted endothelial cells (ECs) were transcriptomically indistinguishable from healthy ECs and lacked an activation signature, while co-opted pericytes expressed a quiescence signature, in contrast to activated pericytes during angiogenesis. Compared with cancer cells during angiogenesis, co-opting cancer cells were phenotypically more diverse and enriched in invasive subpopulations. Together, these data reveal new insight into vessel co-option, with possible implications for the development of therapeutic targets.

2021-06-15 | E-MTAB-9227 | biostudies-arrayexpress

Active protein ubiquitination regulates xylem vessel functionality

Project description:Xylem vessels function in the long-distance conduction of water in land plants. The NAC transcription factor VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a master regulator of xylem vessel cell differentiation in Arabidopsis (Arabidopsis thaliana). We previously isolated seiv (suppressor of ectopic xylem vessel cell differentiation induced by VND7) mutants, which are suppressor mutants of VND7-inducible xylem vessel cell differentiation. Here, we report that the responsible genes for seiv3, seiv4, seiv6, and seiv9 are protein ubiquitination-related genes encoding PLANT U-BOX46 (PUB46), uncharacterized F-BOX protein, PUB36, and UBIQUITIN-SPECIFIC PROTEASE1, respectively. We also found the decreased expression of genes downstream of VND7 and abnormal xylem transport activity in the seiv mutants. Upon VND7 induction, ubiquitinated levels from 492 and 180 protein groups were up- and down-regulated, respectively. Proteins for cell wall biosynthesis and protein transport were ubiquitinated by the VND7 induction, whereas such active protein ubiquitination was not observed in the seiv mutants. We detected the ubiquitination of three lysine residues in VND7: K94, K105, and K260. Substituting K94 with arginine significantly decreased the transactivation activity of VND7, suggesting that the ubiquitination of K94 is crucial for regulating VND7 activity. Our findings highlight the crucial roles of target protein ubiquitination in regulating xylem vessel activity.

2024-10-17 | PXD048830 | Pride

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