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MRNA expression in iPS cells generated by a synthetic self-replicative RNA

ABSTRACT: We generated iPS cells with a synthetic self-replicative RNA that expresses four independent reprogramming factors (OCT4, KLF4, SOX2 and either c-MYC or GLIS1). We performed whole genome RNA sequencing (RNA-seq) of iPS cell clones, parental BJ and HUES9 ES cell controls. All iPS cell clones analyzed by RNA-seq showed unsupervised hierarchical clustering and expression signatures characteristic of human HUES9 ES cells that were highly divergent from parental human fibroblasts.

ORGANISM(S): Homo sapiens

PROVIDER: GSE38265 | GEO | 2014/04/25

SECONDARY ACCESSION(S): PRJNA167504

REPOSITORIES: GEO

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mRNA expression in iPS cells generated by a synthetic self-replicative RNA

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