Colon tissue of Il10-/- mice monoassociated with Escherichia coli NC101 or Escherichia faecalis OG1RF (PAMM-077 array)
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ABSTRACT: We observed that interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM) and monoassociated with E. coli NC101, but not E. faecalis OG1RF, develop tumors. Histologic inflammation is not different in mice monoassociated with either bacterium. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice.
Project description:We observed that interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM) and monoassociated with E. coli NC101, but not E. faecalis OG1RF, develop tumors. Histologic inflammation is not different in mice monoassociated with either bacterium. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice.
Project description:We observed that interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM) and monoassociated with E. coli NC101, but not E. faecalis OG1RF, develop tumors. Histologic inflammation is not different in mice monoassociated with either bacterium. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice. 4 germ-free Il10-/- mice were assayed and used as controls. 4 E. coli and 4 E. faecalis monoassociated mice were experimental samples.
Project description:We observed that interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM) and monoassociated with E. coli NC101, but not E. faecalis OG1RF, develop tumors. Histologic inflammation is not different in mice monoassociated with either bacterium. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice. 4 germ-free Il10-/- mice were assayed and used as controls. 4 E. coli and 4 E. faecalis monoassociated mice were experimental samples.
Project description:Liquid cultures of Enterococcus faecalis OG1RF and OG1RF Δbph were grown in tryptic soy broth without added dextrose (TSB-D) for 2 and 4 hr. At each time point, the transcriptomes were compared to identify differentially expressed genes in the Δbph mutant.
Project description:We observed that deletion of polyketide synthase (pks) from E. coli NC101 reduces its ability to induce tumors in interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM), without altering histologic inflammation. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice. 2 germ-free Il10-/- mice were assayed and used as controls. 3 E. coli NC101 and 3 E. coli NC101-delta-pks monoassociated mice were experimental samples.
Project description:E. faecalis OG1RF cultures were infected with lytic phage VPE25 and proteomic profile of infected cells were compared to uninfected E. faecalis cells using 1D-LC-MS/MS analyses.
Project description:Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis.
Project description:Changes in Enterococcus faecalis OG1RF(pCF10) gene expression at 4 hours post-infection in a rabbit model of subdermal abscess formation were studied using RNA-seq analysis.
Project description:Transcriptional profiling to define the stringent response regulon and significance of basal (p)ppGpp levels in E. faecalis OG1RF. RNA was extracted from four replicate samples of each strain of interest and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from E. faecalis OG1RF cells grown to mid-log.