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Proteomics of phage VPE25 treated Enterococcus faecalis OG1RF relative to untreated controls

ABSTRACT: E. faecalis OG1RF cultures were infected with lytic phage VPE25 and proteomic profile of infected cells were compared to uninfected E. faecalis cells using 1D-LC-MS/MS analyses.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Enterococcus Phage Vpe25 Enterococcus Faecalis Og1rf

SUBMITTER: Fernanda Salvato

LAB HEAD: Manuel Kleiner

PROVIDER: PXD026873 | Pride | 2024-07-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Efaecalis_phage_v2.fasta	Fasta
	Phage_S1_400ng_run3.msf	Msf
	Phage_S1_400ng_run3.raw	Raw
	Phage_S2_400ng_run3.msf	Msf
	Phage_S2_400ng_run3.raw	Raw

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Publications

Enterococcal quorum-controlled protease alters phage infection.

Sheriff Emma K EK Salvato Fernanda F Andersen Shelby E SE Chatterjee Anushila A Kleiner Manuel M Duerkop Breck A BA

bioRxiv : the preprint server for biology 20240511

Increased prevalence of multidrug resistant bacterial infections has sparked interest in alternative antimicrobials, including bacteriophages (phages). Limited understanding of the phage infection process hampers our ability to utilize phages to their full therapeutic potential. To understand phage infection dynamics we performed proteomics on <i>Enterococcus faecalis</i> infected with the phage VPE25. We discovered numerous uncharacterized phage proteins are produced during phage infection of < ...[more]

PMID: 38766208

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