Project description:To investigate the role of TAZ downstream of the abberrant Wnt signaling in CRC cells, we compared the expression profiles of parental SW480 cells (empty vector) transfected with siControl, siTAZ, sibeta-catenin or reconstituted with wild type APC and transfected with siControl Keywords: expression profiling by array We collected RNA from parental SW480 cells (empty vector) transfected with siControl, siTAZ, sibeta-catenin or from SW480 cells reconstituted with wild type APC and transfected with siControl. Cells were left untreated in normal culturing conditions before harvesting. Samples were then processed for total RNA extraction and hybridization on Affymetrix microarrays. Four biological replicas (A, B, C, D) were used for each of the 4 conditions (1: siControl, control cells; 2: siTAZ cells; 3: sibeta-catenin (sibcat) cells; 4.APC reconstituted cells) for a total of 16 samples.
Project description:To investigate the role of TAZ downstream of APC and beta-catenin in mammary epithelial cells cells, we compared the expression profiles of MCF10-T1k (MII) cells transfected with siControl, siAPC, siAPC+siTAZ, sibeta-catenin, or sibeta-catenin+siTAZ. Keywords: expression profiling by array We collected RNA from MII cells transfected with siControl, siAPC, siAPC+siTAZ, sibeta-catenin, or sibeta-catenin+siTAZ. Cells were left untreated in normal culturing conditions before harvesting. Samples were then processed for total RNA extraction and hybridization on Affymetrix microarrays. Four biological replicas (A, B, C, D) were used for each of the 5 conditions (1: siControl, control cells; 2: siAPC cells; 3: siAPC+siTAZ cells; 4: sibeta-catenin (sibcat) cells; 5: sibeta-catenin+siTAZ) for a total of 20 samples.