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Pac1+/+ versus Pac1-/- BMMCs_IgE-DNP 2 h

ABSTRACT: Bone marrow-derived mast cells were differentiated over 4-6 weeks using bone marrow from Pac-1+/+ and Pac1-/- littermate mice. Cell purity was 99% c-kit and Fc epsilon receptor positive as assessed by flow cytometry. Cells were stimulated by Fc epsilon receptor crosslinking using IgE-DNP/HSA for sensitization for 18 hours and DNP-HSA antigen for crosslinking for 2 hours. Gene transcript abundance was determined and scaled to 150 using alogorithms in MicroArray Analysis Suite Software 5.0 (Affymetrix). Keywords: Cpmparison of Pac-1+/+ and Pac-1-/- activated BMMCs

ORGANISM(S): Mus musculus

PROVIDER: GSE3994 | GEO | 2006/01/10

SECONDARY ACCESSION(S): PRJNA104887

REPOSITORIES: GEO

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