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Pac1+/+ versus Pac1-/- in macrophages and mast cells

ABSTRACT: Pac1+/+ versus Pac1-/- in macrophages and mast cells

PROVIDER: PRJNA95111 | ENA |

REPOSITORIES: ENA

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Transcription profiling of mouse macrophaes and mast cells from Pac1+/+ versus Pac1-/- animals

Project description:This SuperSeries is composed of the following subset Series:; GSE3993: Pac1+/+ versus Pac1-/- TG-macrophages_LPS 6h; GSE3994: Pac1+/+ versus Pac1-/- BMMCs_IgE-DNP 2 h Experiment Overall Design: Refer to individual Series

2008-06-12 | E-GEOD-4014 | biostudies-arrayexpress

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Pac1+/+ versus Pac1-/- TG-macrophages_LPS 6h

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Pac1+/+ versus Pac1-/- in macrophages and mast cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcription profiling of mouse bone marrow-derived mast cells were differentiated over 4-6 weeks using bone marrow from Pac-1+/+ and Pac1-/- littermate animals

Project description:Bone marrow-derived mast cells were differentiated over 4-6 weeks using bone marrow from Pac-1+/+ and Pac1-/- littermate mice. Cell purity was 99% c-kit and Fc epsilon receptor positive as assessed by flow cytometry. Cells were stimulated by Fc epsilon receptor crosslinking using IgE-DNP/HSA for sensitization for 18 hours and DNP-HSA antigen for crosslinking for 2 hours. Gene transcript abundance was determined and scaled to 150 using alogorithms in MicroArray Analysis Suite Software 5.0 (Affymetrix). Experiment Overall Design: Two genechips on independent mBMMC cultures was performed. The signal data of the .CEL files for the four Affymetrix 430A chips (2 Pac1+/+ and 2 Pac1â/â) were normalised using the RMA method 49. The ebayes function implemented in the version 1.3.12 of the Limma package (2003) of Bioconductor was used to analyse the data and P-values were adjusted for multiple testing. After conversion from logged values to the original intensities, the data was filtered to remove probe sets with mean, non-logged, intensities below 100. Transcripts with p-values less than 0.05 were considered different from Pac1+/+.

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