Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional Changes of HepG2 cells by VECELL 3-D Culture

ABSTRACT: VECELL 3-D insert is newly developed culture scaffold with collagen-coated ePTFE (expanded polytetrafluoroethylene) mesh. Effects of VECELL 3-D insert on the functionalities of HepG2, human hepatocellular carcinoma cell line, were analyzed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE41270 | GEO | 2015/09/30

SECONDARY ACCESSION(S): PRJNA176479

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Murine host body reactions to implanted scaffolds: non-coat polystyrene scaffold vs. collagen- or PMB-coated scaffold

Project description:This study investigated early host reactions to implanted materials to predict successful tissue regeneration with implant. Three kinds of scaffold, i.e., non-coat, collagen-coated, and PMB-coated porous polystylene scaffolds were implanted subcutaneously in mice dorsal area. Those scaffolds were used as bio-incomopatible materials, appropriate materials for tissue regeneration (bio active), and inappropriate to regenration (bio-inert) scaffolds. Seven days after implantation, scaffolds were explanted and total RNA was isolated from infiltrated host cells into scaffold by laser microdissection. Gene expressions of cells in collagen- and PMB-coated scaffold were normalized using results of non coat scaffold. Genes with more than 2-fold difference between collagen and PMB were picked up and narrowed to related keywords; inflammation, angiogenesis, wound healing, and mcrophage polarization. Among those genes, interluekin (IL)-1beta which promote both inflammation and wound healing was up-regulated in collagen-coated scaffold. On the other hand, IL-10 which suppress both inflammation and wound healing was up-regulated in PMB-coated scaffold. Angiogenesis-promoting genes were up-regulated and angiogenesis suppressve genes were suppressed in collagen. Up-regulation of IL-1b and the angiogenesis-relating genes inside the porous scaffolds are the possibly important factors for controlling tissue regeneration. Three-condition experiment, host cells infiltrated in non coat (reference), collagen-coated, and PMB-coated scaffolds. Two-microarray condition experiments, collagen vs. non coat and PMB coat vs. non coat. Hybridization: 2 replicates. Scanning: 3 replicates. Biological experiments: once.

2014-01-23 | E-GEOD-52053 | biostudies-arrayexpress

Collagen-GAG Biocompatibility Tests

Project description:All mRNA was isolated after 8 hours of culture time in each of three culture conditions. (1) TCPS Plate, (2) Collagen-GAG 2 dimensional coated plate and (3) collagen-GAG three dimensional mesh. Keywords: ordered

2004-11-09 | GSE1937 | GEO

Murine host body reactions to implanted scaffolds: non-coat polystyrene scaffold vs. collagen- or PMB-coated scaffold

2014-01-23 | GSE52053 | GEO

Transcription profiling of human fibroblasts grown on TCPS plates, Collagen-GAG 2 D plate and Collagen-GAG 3D mesh

2008-06-11 | E-GEOD-1937 | biostudies-arrayexpress

Transcription profiling of human fibroblasts grown in petri dishes vs. attached to a tissue engineering scaffold sampled at 7 timepoints.

Project description:A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold material, with human IMR-90 fibroblasts and compared transcript levels with control cells grown on tissue culture polystyrene. Genes involved in cell signaling, extracellular matrix remodeling, inflammation, angiogenesis and hypoxia were all activated in cells on the collagen-GAG mesh. Understanding the impact of a scaffold on attached cells will facilitate the design of improved tissue engineering materials.

2007-10-26 | E-GEOD-6432 | biostudies-arrayexpress

IMR-90 cells: Collagen Mesh Time Course

Project description:A series of chips with some repeated measurments. Each chip represents a pool of 4 collagen/chondroitin sulfate tissue engineering scaffold meshes seeded with 1 x 10^6 IMR-90 Human Fibroblasts. mRNA was isolated at 30 minutes, 1, 2, 4, 8, 12, 24, 48, and 72 hours after seeding. Keywords = tissue engineering mesh, collagen, chondroitin sulfate, biomaterials design Keywords: time-course

2003-09-10 | GSE409 | GEO

Mus musculus

Project description:Murine host body reactions to implanted scaffolds: non-coat polystyrene scaffold vs. collagen- or PMB-coated scaffold

| PRJNA226592 | ENA

Genome-wide analysis of high glucose regulated genes expression in fully differentiated and polarized CaCo-2 Cells

Project description:Cells were grown on collagen coated insert for 21 days under 25mM (high glucose) or 5mM (control) glucose conditions. We investigated relevant target genes that could be regulated by high glucose levels in CaCo-2 cells. We intended to find candidate genes that may play a role for intestinal glucose absorption. Fund Source: The Scientific and Technological Research Council of Turkey Project ID: 214Z217 (T B TAK/ PN:214Z217)

2018-04-14 | GSE97977 | GEO

Transcription profiling of human CD34+ hematopoietic cells grown in suspension culture or in a collegen I matrix

Project description:CD34+ hematopoietic stem/progenitor cells (HSC) reside in the bone marrow in close vicinity to the endosteal bone surface, surrounded by osteoblasts, stromal cells and various extracellular matrix molecules. We utilized a bioartificial matrix containing fibrillar collagen I, the major matrix component of bone, as scaffold for ex vivo expansion of HSCs. CD34+ HSCs were isolated from umbilical cord blood and cultivated within reconstituted collagen I fibrils in presence of FLT3-ligand, SCF and IL-3. After seven days of culture cell number, colony-forming units and gene expression profile of the cultured cells were assessed. Although the total expansion factor of CD34+ cells was slightly lower when cells were cultivated in the collagen I gel, the frequency of colony-forming units (CFU-C) increased compared to control suspension cultures. Gene expression analysis with microarray chip technology revealed the upregulation of more than 50 genes in presence of collagen I. Among them, genes for several growth factors, cytokines and chemokines (e.g. interleukin 8, MIP1-Î±) could be confirmed by quantitative PCR. Furthermore, increased expression of the negative cell-cycle regulator BTG2/TIS21 and an inhibitor of MAP kinase pathway, DUSP2, underline the regulatory role of the extracellular matrix. Together, these data show that the expansion of CD34+ cord blood cells in a culture system containing a three-dimensional collagen I matrix induces a qualitative change in the gene expression profile of cultivated HSCs. Experiment Overall Design: Gene expression profiling of CD34+ hematopoietic cells expanded in a collagen I matrix

2007-12-12 | E-GEOD-3003 | biostudies-arrayexpress

Homo sapiens

Project description:IMR-90 cells: Collagen Mesh Time Course

| PRJNA104715 | ENA