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Hog1 bypasses stress-mediated downregulation of transcription by PolII redistribution and chromatin remodeling

ABSTRACT: Hog1 bypasses stress-mediated downregulation of transcription by PolII redistribution and chromatin remodeling Keyword: genetic modification

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE41451 | GEO | 2012/10/30

SECONDARY ACCESSION(S): PRJNA177031

REPOSITORIES: GEO

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Hog1 bypasses stress-mediated downregulation of transcription by PolII redistribution and chromatin remodeling

Project description:Hog1 bypasses stress-mediated downregulation of transcription by PolII redistribution and chromatin remodeling Keyword: genetic modification Three independent 200 ml cultures of wild-type strain were grown to mid-log phase in YPD rich medium at 30ºC

2012-10-30 | E-GEOD-41451 | biostudies-arrayexpress

Hog1 bypasses stress-mediated down-regulation of transcription by PolII redistribution and chromatin remodeling

Project description:Cells are subjected to dramatic changes on gene expression upon environmental changes. Stress causes a general down-regulation of gene expression together with the induction of a set of stress-responsive genes. Genome wide localisation studies showed major changes on Pol II localisation towards stress-responsive genes in contrast to housekeeping genes. Pol II relocalisation requires of the Hog1 SAPK, which also associates at stress-responsive loci. Chromatin structure was not significantly altered upon stress except for those genes that displayed Hog1 association. Together, Hog1 serves to bypass the general down-regulation of gene expression by targeting RNA Pol II machinery and inducing chromatin remodeling at stress-responsive loci.

2012-10-30 | GSE41494 | GEO

Hog1 bypasses stress-mediated down-regulation of transcription by PolII redistribution and chromatin remodeling

2012-10-30 | E-GEOD-41494 | biostudies-arrayexpress

Saccharomyces cerevisiae

Project description:Hog1 bypasses stress-mediated downregulation of transcription by PolII redistribution and chromatin remodeling

| PRJNA177031 | ENA

Saccharomyces cerevisiae

Project description:Hog1 bypasses stress-mediated down-regulation of transcription by PolII redistribution and chromatin remodeling

| PRJNA177335 | ENA

Hog1 bypasses stress-mediated down-regulation of transcription by RNA polymerase II redistribution and chromatin remodeling.

Project description:Cells are subjected to dramatic changes of gene expression upon environmental changes. Stress causes a general down-regulation of gene expression together with the induction of a set of stress-responsive genes. The p38-related stress-activated protein kinase Hog1 is an important regulator of transcription upon osmostress in yeast.Genome-wide localization studies of RNA polymerase II (RNA Pol II) and Hog1 showed that stress induced major changes in RNA Pol II localization, with a shift toward stress-responsive genes relative to housekeeping genes. RNA Pol II relocalization required Hog1, which was also localized to stress-responsive loci. In addition to RNA Pol II-bound genes, Hog1 also localized to RNA polymerase III-bound genes, pointing to a wider role for Hog1 in transcriptional control than initially expected. Interestingly, an increasing association of Hog1 with stress-responsive genes was strongly correlated with chromatin remodeling and increased gene expression. Remarkably, MNase-Seq analysis showed that although chromatin structure was not significantly altered at a genome-wide level in response to stress, there was pronounced chromatin remodeling for those genes that displayed Hog1 association.Hog1 serves to bypass the general down-regulation of gene expression that occurs in response to osmostress, and does so both by targeting RNA Pol II machinery and by inducing chromatin remodeling at stress-responsive loci.

| S-EPMC3580498 | biostudies-literature

Hog1 controls global reallocation of RNA Pol II upon osmotic shock

2012-07-15 | GSE38208 | GEO

Hog1 ChIP Chip

Project description:Yeast Hog1-3HA ChIP-Chip in triplicate Keywords: ChIP-Chip

2006-05-26 | GSE4862 | GEO

Hog1 ChIP Chip

Project description:Yeast Hog1-3HA ChIP-Chip in triplicate Keywords: ChIP-Chip Yeast Hog1 ChIP-Chip was performed in MAP51 (3HA-Hog1) in triplicate using anti-HA (12CA5 monoclonal antibody)

2010-07-01 | E-GEOD-4862 | biostudies-arrayexpress

Hog1 controls global reallocation of RNA Pol II upon osmotic shock

Project description:When challenged with osmotic shock, S. cerevisiae induces hundreds of genes, despite a global reduction in transcriptional capacity. The mechanisms that regulate this rapid reallocation of transcriptional resources are not known. Here we show that redistribution of RNA Pol II upon stress requires the stress-responsive MAP kinase Hog1. We find that Hog1 and RNA Pol II co-localize to open reading frames that bypass global transcriptional repression, and that these targets are specified by two osmotic stress-responsive transcription factors. The combination of reduced global transcription with a gene-specific override mechanism allows cells to rapidly switch their transcriptional program in response to stress. ChIP-sequencing of S. cervisiae RNA Pol II, Hog1, Sko1 and Hot1 Processed data file descriptions: ORFcounts.txt: table of summed ChIP-seq reads that align to each ORF (normalized by reads per kilobase per million) promoter_counts.txt: table of summed ChIP-seq reads that align to each promoter (1kb upstream, normalized by reads per kilobase per million) downstream_counts.txt: table of summed ChIP-seq reads that align 3' regions (50-500 bp downstream, normalized by reads per kilobase per million) Sko1_peak_list.txt: table of peaks found by PeakSeq Hot1_peak_list.txt: table of peaks found by PeakSeq

2012-07-15 | E-GEOD-38208 | biostudies-arrayexpress

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