Chromatin In Situ Proximity (ChrISP): Single-cell analysis of chromatin proximities at a high resolution
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ABSTRACT: Current techniques analysing chromatin structures are hampered by either poor resolution of individual cells or high resolution in only large cell populations. Here we describe a new method, ChrISP,which reproducibly scores for chromatin fiber proximities in 3D in single cells with a resolution of < 170 A. Moreover, ChrISP visualizes higher order structures of individual chromosomes in relation to structural hallmarks of interphase nuclei beyond the resolution of the light microscope.
ORGANISM(S): Homo sapiens
PROVIDER: GSE42009 | GEO | 2014/02/20
SECONDARY ACCESSION(S): PRJNA178758
REPOSITORIES: GEO
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