Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global microRNA profiling of aTC1-6 and bTC1 before and after treatment with cytokines

ABSTRACT: The study sought to determine the global miRNA profiles of mouse pancreatic cell lines aTC1-6 and bTC1 at steady state and after treatment with a cocktail of pro-inflammatory cytokines (IL1b; IFNg and TNFa) for a time course of 24 and 48 hours. Inflammatory cocktail contains IL1b 50 IU/ml; IFNg 1000 IU/ml; TNFa 1000 IU/ml. MicroRNA expression profiles in both cell lines during the different experimental conditions were determined using TaqMan® Rodent miRNA A+B Cards Set v2.0 (TLDA, Life Technologies). MicroRNA profiles were measured in 3 independent biological replicates.

ORGANISM(S): Mus musculus

PROVIDER: GSE42970 | GEO | 2012/12/18

SECONDARY ACCESSION(S): PRJNA183965

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Global microRNA profiling of aTC1-6 and bTC1 before and after treatment with cytokines

Project description:The study sought to determine the global miRNA profiles of mouse pancreatic cell lines aTC1-6 and bTC1 at steady state and after treatment with a cocktail of pro-inflammatory cytokines (IL1b; IFNg and TNFa) for a time course of 24 and 48 hours. Inflammatory cocktail contains IL1b 50 IU/ml; IFNg 1000 IU/ml; TNFa 1000 IU/ml. MicroRNA expression profiles in both cell lines during the different experimental conditions were determined using TaqManM-BM-. Rodent miRNA A+B Cards Set v2.0 (TLDA, Life Technologies). MicroRNA profiles were measured in 3 independent biological replicates. qRT-PCR analysis of microRNAs of aTC1-6 treated with cytokines for 24 and 48 h and of their respective not-treated controls as well as of M-NM-2TC1 cells not treated with cytokines. 180 ng RNA of each sample was reverse transcribed through MegaplexM-bM-^DM-" RT Rodent Primer Pool sets A and B, and preamplified through MegaplexM-bM-^DM-" PreAmp Rodent Primer Pool sets (LifetechnologiesM-bM-^DM-"). Complementary DNA (cDNA) was amplified usingTaqManM-BM-. Rodent miRNA A+B Cards Set v2.0 (Life TechnologiesM-bM-^DM-") with TaqMan Universal PCR Master Mix on an ABI 7900HT Sequence Detection System.

2012-12-18 | E-GEOD-42970 | biostudies-arrayexpress

NGS5439 - BMDMs cytokine response

Project description:mouse primary BMDMs were stimulated with IFNg, IFNg + hypoxia, IFNg + IL1b, IFNg + LPS, IFNg +TNFa for 18h

2024-08-14 | GSE268353 | GEO

Bulk RNAseq of AML and endothelial cells in untreated and cytokine-treated conditions

Project description:We report the use of bulk RNAseq of AML and endothelial cell lines in four conditions (1. Untreated; 2. IFNg - 10 ng/ml; 3. TNFa - 10 ng/ml; 4. IFNg and TNFa - 10 ng/ml) in order to identify genes that are differentially expressed between the two cell types, both at baseline and in an inflammatory microenvironment.

2021-10-21 | GSE159991 | GEO

NGS5523 - ATAC-seq WTAP and ZC3H13 KOs in hMDMS

Project description:human primary macropahges (hMDMs) were genetically perturbed for WTAP and ZC3H13. ATAC-seq was performed 18h post IFNg (2ng/ml) or TNFa (10ng/ml) stimulation

2024-08-14 | GSE270634 | GEO

Identification of unique cell type responses in pancreatic islets to stress

Project description:We single cell sequenced multiplexed primary human islets treated ex vivo with ER and inflammatory chemicals, indexed cell type specific stress responses, found genes to regulate in SC-islets to reduce apoptosis. We conducted multiomic ATAC+RNA sequencing on human islets treated with BFA, DMSO, IL1B+IFNG+TNFa, or PBS

2024-05-06 | GSE237448 | GEO

NGS5522 - hMDMS response to IFNg and TNFa

Project description:human primay macrophages (hMDMs) were unstimulated, stimulated with IFNg or costimulated with IFNg and TNFa for 18h

2024-08-14 | GSE268354 | GEO

Genome-wide analysis of the transcriptional profile of IL1b/TNFa-treated and EAE astrocytes in B6 mice.

Project description:We identify pathways regulated by IL1b/TNFa ICV injection and EAE in astrocytes

2020-02-12 | GSE137314 | GEO

Proteomic analyisis of cultured human podocytes stimulated with TNFa - cell pellet

Project description:TNFa stimulated human cultured podocytes compared with unstimulated (vehicle control (VC)) podocytes. Incubation of podocytes with high concentrations of TNF alpha led to an overexpression of ICAM1 in particular. We studied this system as a comparator for organoids also stimulated with the same concentration of TNFa. Four conditions (6 replicates each) 1) treatment (5 ng/mL TNFa) for 24 hours 2) control (PBS) for 24h 3) treatment (5 ng/mL TNFa) for 48 hours 4) control (PBS) for 48h

2023-05-19 | PXD032107 | Pride

Proteomic analyisis of cultured human podocytes stimulated with TNFa - supernatant

2023-05-19 | PXD032130 | Pride

Design of a gene signature to analyze the proinflammatory and the profibrotic polarization of murine fibroblasts and validation in an experimental murine model of scleroderma.

Project description:Effect of TGFb1, TNFa, IL6-IL6R, IL1b on gene expression in Mouse Embryonnic Fibrobroblasts (3T3) using RNA microarrays.

2022-07-20 | GSE191223 | GEO