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Affy_med_2012_03-Characterization of new phytostimulatrices molecules for the improvement of plants products and crop resistance to diseases

ABSTRACT: affy_med_2012_03 - affy_med_2012_03 - Aphanomyces euteiches is a major legume parasite related to S. parasitica. Analysis of its cell wall showed the presence of an unsusual high content in GlcNAc, representing 10% of the total carbohydrates. Interestingly, this compound does not form crystalline chitin, but amorphous chitosaccharides. The aim goal of this study is to compare the response to a preparation of crab shell chitin chitooligosaccharides with a mean DP of 7 and to A.euteiches chitosaccharides at a transcriptomic level. This comparison will be done on Medicago truncatula wild type compared to the nfp-1 and nfp-2 mutants. - 7-day-old A17 wild type or mutant nfp-1 or nfp-2 plants were grown in vitro on liquid M medium. The entire roots were then treated (or not = controls) with a preparation of crab shell chitin chitooligosaccharides of a mean DP of 7 or with chitosaccharides from A. euteiches cell wall, names fraction P2F1, and harvested after 4 hours of incubation. Three independent repeats were performed.

ORGANISM(S): Medicago sativa Sinorhizobium meliloti Medicago truncatula

PROVIDER: GSE43839 | GEO | 2013/12/01

SECONDARY ACCESSION(S): PRJNA188666

REPOSITORIES: GEO

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affy_med_2012_03-Characterization of new phytostimulatrices molecules for the improvement of plants products and crop resistance to diseases

Project description:affy_med_2012_03 - affy_med_2012_03 - Aphanomyces euteiches is a major legume parasite related to S. parasitica. Analysis of its cell wall showed the presence of an unsusual high content in GlcNAc, representing 10% of the total carbohydrates. Interestingly, this compound does not form crystalline chitin, but amorphous chitosaccharides. The aim goal of this study is to compare the response to a preparation of crab shell chitin chitooligosaccharides with a mean DP of 7 and to A.euteiches chitosaccharides at a transcriptomic level. This comparison will be done on Medicago truncatula wild type compared to the nfp-1 and nfp-2 mutants. - 7-day-old A17 wild type or mutant nfp-1 or nfp-2 plants were grown in vitro on liquid M medium. The entire roots were then treated (or not = controls) with a preparation of crab shell chitin chitooligosaccharides of a mean DP of 7 or with chitosaccharides from A. euteiches cell wall, names fraction P2F1, and harvested after 4 hours of incubation. Three independent repeats were performed. 27 arrays - Medicago; normal vs ems mutant comparison

2013-12-01 | E-GEOD-43839 | biostudies-arrayexpress

Medicago truncatula roots infected (or not) with the oomycete Aphanomyces euteiches

Project description:The objective of this study was to evaluate the effect of the oomycete Aphanomyces euteiches (strain ATCC201684) on the legume model Medicago truncatula (F83005-5 line) transcritpome.

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Transcription profiling of Medicago roots from MtTi2i vs control after infection with Aphanomyces euteiches

Project description:Comparsion of root transcriptome profiles after Aphanomyces euteiches infections between control and MtTi2i-lines

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Sesquiterpenes produced in roots of Medicago truncatula play a role in defense against Aphanomyces euteiches

Project description:Inoculation of Medicago truncatula roots with zoospores of the oomycetic pathogen Aphanomyces euteiches leads to rapid upregulation of a gene encoding a sesquiterpene synthase followed by release of sesquiterpenes that may have a defense function against A. euteiches.

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Aphanomyces euteiches

Project description:Aphanomyces euteiches overview

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affy_med_2011_10- Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the lyr3-1 and lyr3-2 mutants

Project description:affy_med_2011_10 - affy_med_2011_10 - LYR3 encodes a lysin motif-receptor-like kinase (LysM-RLK) that consists of an extracellular ligand-binding domain composed of three LysM domains, a single transmembrane domain and a cytoplasmic serine/threonine kinase domain. A reverse genetic approach was undertaken to study the role of this gene in the pathosystem Medicago truncatula – Aphanomyces euteiches (Ae). Results suggest that lyr3-1 and lyr3-2 mutants are more susceptible to the root pathogen Aphanomyces euteiches than the A17 wild type (WT) line. In this study, we want to compare the early plant responses, 3 days post inoculation (dpi), between WT and the lyr3-1 and lyr3-2 mutants, in order to identify LYR3-dependent gene networks during infection.-Fourteen days old A17, lyr3-1 or lyr3-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with A. euteiches zoospores (105/ml) and harvested three days after inoculation. Three independent repeats were performed.

2014-02-28 | GSE43715 | GEO

The Vibrio cholerae chitin utilization program

Project description:This SuperSeries is composed of the following subset Series: GSE3101: Chitin oligosaccharide induction GSE3102: Crab shell attachment GSE3103: Chitin sensor Abstract: Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GlcNAc)(2-6), GlcNAc, or the glucosamine dimer (GlcN)2 identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc)(2-6) gene set, including a (GlcNAc)2 catabolic operon, two extracellular chitinases, a chitoporin, and a PilA-containing type IV pilus, designated ChiRP (chitin-regulated pilus) that confers a significant growth advantage to V. cholerae on a chitin surface; (ii) GlcNAc causes the coordinate expression of genes involved with chitin chemotaxis and adherence and with the transport and assimilation of GlcNAc; (iii) (GlcN)2 induces genes required for the transport and catabolism of nonacetylated chitin residues; and (iv) the constitutively expressed MSHA pilus facilitates adhesion to the chitin surface independent of surface chemistry. Collectively, these results provide a global portrait of a complex, multistage V. cholerae program for the efficient utilization of chitin. Refer to individual Series

2006-03-07 | E-GEOD-4388 | biostudies-arrayexpress

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Project description:Aphanomyces euteiches Genome sequencing

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Transcription profiling of Vibrio cholerae strain N16961 was incubated with crab shell in artificial sea water media for 24 hours

Project description:Strain N16961 was incubated with crab shell in artificial seawater media for 24 hours. cDNA from 1 ug RNA was labeled with Cy3 (planktonic bacteria) and Cy5 (crab attached bacteria).

2005-07-21 | E-SMDB-2238 | biostudies-arrayexpress

affy_med_2011_10- Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the lyr3-1 and lyr3-2 mutants

Project description:affy_med_2011_10 - affy_med_2011_10 - LYR3 encodes a lysin motif-receptor-like kinase (LysM-RLK) that consists of an extracellular ligand-binding domain composed of three LysM domains, a single transmembrane domain and a cytoplasmic serine/threonine kinase domain. A reverse genetic approach was undertaken to study the role of this gene in the pathosystem Medicago truncatula M-bM-^@M-^S Aphanomyces euteiches (Ae). Results suggest that lyr3-1 and lyr3-2 mutants are more susceptible to the root pathogen Aphanomyces euteiches than the A17 wild type (WT) line. In this study, we want to compare the early plant responses, 3 days post inoculation (dpi), between WT and the lyr3-1 and lyr3-2 mutants, in order to identify LYR3-dependent gene networks during infection.-Fourteen days old A17, lyr3-1 or lyr3-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with A. euteiches zoospores (105/ml) and harvested three days after inoculation. Three independent repeats were performed. 18 arrays - Medicago; normal vs mutant comparison,near isogenic lines (hif)

2014-02-28 | E-GEOD-43715 | biostudies-arrayexpress

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