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In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera

ABSTRACT: Examining of the global gene expression changes in cultured human pancreatic cancer cells effected by the extract of A. aspera leaves. Comparison of cells treated for 24, 48 and 72 hours with the respective controls (untreated) allowed to select key genes that may be of interest as a response markers.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44290 | GEO | 2013/12/01

SECONDARY ACCESSION(S): PRJNA189326

REPOSITORIES: GEO

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Achyranthes aspera leaf extract modulates the expression of genes involved in immune response and embryonic development etc in vitro in cultured human pancreatic cancer cell line MIA PaCa-2 (CRL1420).

Project description:Examining of the global gene expression changes in cultured human pancreatic cancer cells effected by the extract of A. aspera leaves. Comparison of cells treated for 24, 48 and 72 hours with the respective controls (untreated) allowed to select key genes that may be of interest as a response markers. Two-condition experiment, treated vs. untreated cells at 3 time points. Biological replicates: 6 control and 6 treated replicates for 24 hours time point; 4 control and 4 treated replicated for 48 hours time point; 2 control and 2 treated replicates for 72 hours time point.

2013-12-01 | E-GEOD-44290 | biostudies-arrayexpress

Transcriptional analyses of maize leaves in response to high‐density planting

Project description:Maize (Zea mays L.) is one of the major cereal crops worldwide. Increasing planting density is an effective way to improve crop yield. However, plants grown under high-density conditions compete for water, nutrients, and light, which often leads to changes in productivity. To date, few studies have determined the transcriptomic differences in maize leaves in response to different planting densities. This study examined the whole-genome expression patterns in the leaves of maize planted under high and low densities to identify density-regulated genes. Leaves at upper, ear, and lower stem nodes were collected at the grain-filling stage of the maize hybrid Xianyu335 grown under low-density planting and high-density planting. In total, 72, 733, and 1,739 differentially expressed genes (DEGs) were identified in the respective upper, ear, and lower leaves under HDP. Upregulated and downregulated DEGs in the upper and lower leaves were similar in number, whereas upregulated DEGs in the ear leaves were significantly higher in number than the downregulated DEGs. Functional analysis indicated that genes responding to HDP-related stresses were mediated by pathways involving four phytohormones responsible for metabolism and signaling, osmoprotectant biosynthesis, transcription factors, and fatty acid biosynthesis and protein kinases, which suggested that these pathways are affected by the adaptive responses mechanisms underlying the physiological and biochemical responses of the leaves of maize planted at high density.

2020-06-29 | GSE152107 | GEO

P. syringae pv. phaseolicola NPS3121 responses to bean leaf extract

Project description:Many genes involve in pathogenicity and virulence are induced only in plant or in the presence of host components. Bean leaf extract was obtained from healthy bean leaves. In this work we investigated the effect of bean leaf extract on the transcriptomic profile of the bacterium, when grown at low temperature in minimal medium with or without extract from healthy bean leaves.

2009-03-03 | GSE14998 | GEO

Effect of Annona Muricata Leaves on Colorectal Cancer Patients and Colorectal Cancer Cells

Project description:Annona muricata L., include the leaves, is found to contain biologically active Annonaceous acetogenins and plant polyphenols that are important components of human diet and a number of them are considered to have chemopreventive and therapeutic properties against cancer. To confirm previous findings in in vitro, animal study and traditionally use, a human, ex vivo and in vitro studies were conducted to evaluate the effects of consecutive ingestion of A. muricata leaves extract for eight weeks.

| 2189667 | ecrin-mdr-crc

Modulation of apple leaf trancriptome by tomato cutin monomer extract

Project description:Investigation of whole genome gene expression level changes in leaves of apple seedlings (Golden delicious) 3 days after treatment by tomato cutin monomer extract (CME) versus formulation blank (FB). CME is a formulated extract enriched of hydroxy fatty acids from tomato cuticle

2024-02-09 | GSE245564 | GEO

In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera.

Project description:Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties. We have systematically validated the in vitro and in vivo anticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34? ? g/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ? 0.01 and FC of ?±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis-inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use of A. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.

| S-EPMC3880711 | biostudies-literature

kaempferitrin-induced secretome analyses in rat CTX TNA2 astrocytic cell line

Project description:Kaempferitrin is extracted at significant quantity from leaves of Cinnamomum osmophloeum. People consuming extract containing kaempferitrin often happen to be of high risk for diabetes and neurodegenerative diseases. For kaempferitrin to be used every day for them, the systematic effect, especially on immune system and on the CNS, needs to be addressed. In this project we used astrocytic cell line as a model to test the differentially regulated secretomes, to test kaemfperitrin effect on CNS glia.

2017-11-24 | PXD002814 | Pride

Real-time quantitative PCR analysis of Hepatic cell lines infected with HCV

Project description:huh7.5 hepatic cell lines were infected with HCV. RNA and Protein were extract after 72 hours and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group.

2014-03-07 | GSE55677 | GEO

Transcriptome Analysis of Populus x canadensis in Response to Drought Stress

Project description:To investigate the response of poplar hybrids to drought, leaves were collected from plants to which water was suspended for 8 and 13 days. After measuring the respective relative water content, RNAs were isolated from leaves of moderately and severely droughted plants and from control plants, and Illumina RNA sequencing was performed to analyze RNA synthesis in these tissues.

2015-01-21 | GSE64044 | GEO

Cold stress in rice - Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

Project description:Rice seedlings were exposed to low temperature and leaves were harvested after 0, 48, 72 and 96 hours. Proteins were analysed using label-free quantitative proteomics.

2011-03-03 | PRD000361 | Pride

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