Molecular characterization of the apical organ in the anthozoan Nematostella vectensis

ABSTRACT: The aim of the experiment was the identification of genes that are expressed in the apical organ of the sea anemone Nematostella vectensis. To achieve this, animals were generated that either lack the apical organ (injected with a morpholino antisense oligonucleotide to block the function of NvFGFa1) or have an expanded apical organ (injected with a morpholino antisense oligonucleotide to block the function of NvFGFa2, see Rentzsch et al., Development, 2008). Eggs from the same batch were injected after fertilization, (MO NvFGFa1 at 0.5uM, MO NvFGFa2 at 0.25uM) and planula larvae with the expected phenotype and without general developmental abnormalities were selected after 72 hours of development at room temperature (22°C), and placed in RNAlater Solution (Ambion) for subsequent extraction of RNA. Three conditions were isolated: embryos injected with NvFGFa1 MO, embryos injected with NvFGFa2 MO and non-injected embryos. Total RNA was extracted with the RNAqueous kit (Ambion), following the manufacturer’s protocol, and temporarily stored at -80°C, in order to accumulate the quantity necessary for the hybridization. The isolated total RNA for each condition was pooled and split into to two tubes and shipped on dry ice to Nimblegen in Iceland. The injections and the isolation of total RNA were performed from May – August 2009 at the Sars Centre in Bergen, Norway.

ORGANISM(S): Nematostella vectensis

SUBMITTER: Fabian Rentzsch 

PROVIDER: E-MTAB-3004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress