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Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [methylation profiling]

ABSTRACT: We treated melanoma cells with BRAF mutation with BRAF inhibitor and screened for BRAF inhibitor resistant cells. We extracted DNA from parental cells and resistant cell lines. We compared the DNA methylation via Illumina 450K Methylation Array

ORGANISM(S): Homo sapiens

PROVIDER: GSE45647 | GEO | 2014/12/17

SECONDARY ACCESSION(S): PRJNA195562

REPOSITORIES: GEO

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Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [methylation profiling]

Project description:We treated melanoma cells with BRAF mutation with BRAF inhibitor and screened for BRAF inhibitor resistant cells. We extracted DNA from parental cells and resistant cell lines. We compared the DNA methylation via Illumina 450K Methylation Array Bisulphite converted DNA from the 4 specimens was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

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