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Expression Profiling in Selenite-induced Proliferation of HuH7 cells

ABSTRACT: Experimental studies confirmed that seleium induced a global change of gene expression in HuH7 cells. Several novally induced secretory growth factors may contribute to the autocrine mode of growth elicited by selenium treatment. Keywords: Treatment

ORGANISM(S): Homo sapiens

PROVIDER: GSE4593 | GEO | 2008/12/25

SECONDARY ACCESSION(S): PRJNA94449

REPOSITORIES: GEO

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Expression Profiling in Selenite-induced Proliferation of HuH7 cells

Project description:Experimental studies confirmed that seleium induced a global change of gene expression in HuH7 cells. Several novally induced secretory growth factors may contribute to the autocrine mode of growth elicited by selenium treatment. Keywords: Treatment HuH7 were cultured in 0.1 Î¼M sodium selenite-containing medium for 15 passages (SELT) or in serum-free medium for 30 h (SF30) or in the regular complete medium containing 10 % fetal calf serum (HUH7) for 30 h. The total RNA were reverse transcried and hybridized to Human 8K-1 (Egenomix Technology Corp., Taiwan). The hybridization time and temperature was 16 h and 62â??, respectively. SELT and HUH7 samples were labeled with Cy5 while SF30 sample was labeled with Cy3. Local background correction and interchip global normalization were accomplished by GenePix Pro 5.0 (Molecular Devices, Sunnyvale, CA ). Genes with differential up- or down-regulation by 1.5 fold with respect to the SF30 cells were obtained using GeneSpring software 7.0 (Silicon Genetics, Agilent Technologies).

2010-06-30 | E-GEOD-4593 | biostudies-arrayexpress

shControl Huh7 and shARID1A Huh7 cells without or with rapamycin treatment

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DNA microarray analysis of Huh7 and JMJD5KO Huh7 cells.

Project description:To understand the roles of JMJD5 in liver cells, we performed DNA microarray analysis by using JMJD5KO Huh7 cells. We noticed that several transcriptional factors involved in differentiation to hepatocytes were down-regulated in JMJD5KO Huh7 cells compared to parent Huh7 cells..

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RNA seq data of Hep3B-control, Hep3B-sertraline, Hep3B-XL413, Hep3B-XL413-sertraline, Huh7-control, Huh7-sertraline, Huh7-XL413, Huh7-XL413-sertraline cells

Project description:Hep3B and Huh7 cells pre-treated with XL413 for 10 days to induce senescence prior to sertraline treatment for 24 hours. For RNA sequencing, the library was prepared using TruSeq RNA sample prep kit according to the manufacturer’s protocol (Illumina). Gene set enrichment analysis was performed using gene set enrichment analysis software.

2018-10-16 | GSE121277 | GEO

Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

Project description:The yeast S. cerevisiae does not require selenium for growth, so it is an excellent model to investigate the toxicity of this element without interference from its requirement as growth factor as occurs in animal cells. Exposure to selenite interferes with the yeast iron metabolism. Aft2 is a transcription factor related (with Aft1) to maintenance of iron homeostasis. We have found that aft2 cells are highly sensitive to selenite (a common environmental source for selenium). In these experiments we investigate the transcriptional response to a sub-lethal dose of selenite in wild type and aft2 cells. Our results show that the aft2 mutation strongly potentiates the transcriptional response to selenite, showing induction of many genes related to the responses to oxidative stress and DNA damage.

2015-12-31 | GSE70835 | GEO

Expression levels of mature miRNAs from Huh7 cell treated with ROCK inhibitor

Project description:We used microarray to determine the miRNA whose expression was changed at 12 hours after Y27632 treatment Two-condition experiment; Huh7 control vs Huh7 treated with Y27632 for 12 hours

2014-04-21 | E-GEOD-33876 | biostudies-arrayexpress

RNAseq of the effect of selenite and coated selenium nanoparticles against spheroids of SKOV-3 and OVCAR-3 ovarian cancer cell lines

Project description:Analysis of transcriptome changes in SKOV-3 and OVCAR-3 spheroids following selenite or coated selenium nanoparticles treatment at sublethal doses for 24 hrs

2023-02-24 | GSE149397 | GEO

ATAC-seq in shControl Huh7 and shARID1A Huh7 cells without or with rapamycin treatment

Project description:ATAC-seq in shControl Huh7 and shARID1A Huh7 cells without or with rapamycin treatment

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RNA-seq in shControl Huh7 and shARID1A Huh7 cells without or with rapamycin treatment

Project description:RNA-seq in shControl Huh7 and shARID1A Huh7 cells without or with rapamycin treatment

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Transcriptome of Pseudomonas putida KT2440 in response to selenite

Project description:The bacterium Pseudomonas putida KT2440 has the ability to reduce selenite forming nanoparticles of elemental selenium. This is the transcriptome of the organism when cultured in the presence of selenite.

2022-10-01 | GSE214391 | GEO

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