Methylation profiling

Dataset Information

0

Genome-wide promoter methylation analysis of human osteoarthritis


ABSTRACT: Osteoarthritis (OA) is a chronic disease of the joint characterized by a progressive degradation of articular cartilage and subchondral bone. In healthy tissue, specialized cells called chondrocytes are regulating a balanced cartilage catabolism and anabolism. By contrast osteoarthritic joints are characterized by a dramatic increase of cartilage catabolism, due to changes of gene expression patterns within chondrocytes. To identify potential epigenetic differences regulating this process a genome-wide methylation screening of paired unaffected and osteoarthritic knee cartilage samples was performed. Therefore samples of macroscopic arthritic and non-arthritic cartilage areas of the femoral condyle of five female patients were collected and DNA isolation was performed. For being able to investigate methylation changes on a genome-wide scale using only limited amounts of DNA a specific amplification protocol for mainly methylated DNA has been established, based on combinations of different methylation-sensitive and –independent restriction digestions. The amplified DNA was then labeled and hybridized onto Agilent “Human Promoter Whole Genome” microarrays. A random variance t-test for paired (per patient) samples was performed, identifying 1214 differentially methylated genetic targets between arthritic and non-arthritic samples. The biological relevance of these genes was then further investigated via Gene Ontology (GO) and KEGG pathway analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE48422 | GEO | 2014/07/01

SECONDARY ACCESSION(S): PRJNA209998

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-07-01 | E-GEOD-48422 | biostudies-arrayexpress
2013-04-01 | GSE43269 | GEO
2013-04-01 | E-GEOD-43269 | biostudies-arrayexpress
2016-07-01 | E-GEOD-73626 | biostudies-arrayexpress
2015-09-02 | GSE63695 | GEO
2013-01-31 | GSE43923 | GEO
2013-01-31 | E-GEOD-43923 | biostudies-arrayexpress
2015-10-06 | GSE73746 | GEO
2013-04-01 | GSE43191 | GEO
2015-10-06 | GSE73749 | GEO