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Comparing control and TGF-beta treated cord blood-derived human mast cells

ABSTRACT: Cord blood-derived stem cells were in vitro cultured in the presence of 40 ng/ml SCF, 20 ng/ml IL6 and 2 microM lysophosphatidic acid for 5 week. Then mast cells were magnetically isolated and further cultured for 4 days in the presence or absence of 2 ng/ml TGF-betaI. Total RNA was isolated and processed according to the Agilent Low-input RNA Linear Amplification Kit and microarray hybridization protocol. Keywords: Agilent Whole Human Genome, dual channel, LogRatio values are log(10) values

ORGANISM(S): Homo sapiens

PROVIDER: GSE4906 | GEO | 2006/06/23

SECONDARY ACCESSION(S): PRJNA95905

REPOSITORIES: GEO

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