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Gene regulation by Pax6 in the lens placode - E9.5 (1)

ABSTRACT: Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE49227 | GEO | 2013/09/01

SECONDARY ACCESSION(S): PRJNA213320

REPOSITORIES: GEO

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Gene regulation by Pax6 in the lens placode - E10.25

2013-09-23 | GSE49216 | GEO

Gene regulation by Pax6 in the lens placode - E9.5 (1)

Project description:Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays. Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues (left and right eyes) from each embryo.

2013-09-01 | E-GEOD-49227 | biostudies-arrayexpress

Gene regulation by Pax6 in the lens placode - E9.5 (2)

2013-09-01 | GSE49228 | GEO

Gene regulation by Pax6 in the lens placode - E9.5 (3)

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Gene regulation by Pax6 in the lens placode - E10.25

Project description:Wild type or Pax6 fx/fx; Le-Cre-positive surface ectoderm from E10.25 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays. Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues from one embryo.

2013-09-23 | E-GEOD-49216 | biostudies-arrayexpress

Gene regulation by CBP-p300 in the lens placode - E9.5

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Gene regulation by Pax6 in the lens placode - E9.5 (3)

Project description:Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.

2013-09-01 | E-GEOD-49229 | biostudies-arrayexpress

Gene regulation by Pax6 in the lens placode - E9.5 (2)

Project description:Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.

2013-09-01 | E-GEOD-49228 | biostudies-arrayexpress

Gene regulation by CBP-p300 in the lens placode - E9.5

Project description:Wild type or CBP/p300 fx/fx; Le-Cre-positive (CBP/p300-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.

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Gene expression at E9.5 after deletion of type I BMP receptors in the mouse lens placode

Project description:The type I BMP receptors, Bmpr1a and Acvr1 were conditionally deleted from the mouse lens placode with Le-Cre. The heads of wild type and knockout E9.5 mouse embryos were laser micodissected to isolate the lens ectoderm from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN Ovation Pico WTA system kit. cDNA was biotinylated and hybridized to Illumina Mouse6 V1.1 bead arrays.

2015-03-09 | GSE64005 | GEO

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