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Gene regulation by Pax6 in the lens placode - E9.5 (3)

ABSTRACT: Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: David Beebe 

PROVIDER: E-GEOD-49229 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The mechanism of lens placode formation: a case of matrix-mediated morphogenesis.

Huang Jie J   Rajagopal Ramya R   Liu Ying Y   Dattilo Lisa K LK   Shaham Ohad O   Ashery-Padan Ruth R   Beebe David C DC  

Developmental biology 20110421 1

Although placodes are ubiquitous precursors of tissue invagination, the mechanism of placode formation has not been established and the requirement of placode formation for subsequent invagination has not been tested. Earlier measurements in chicken embryos supported the view that lens placode formation occurs because the extracellular matrix (ECM) between the optic vesicle and the surface ectoderm prevents the prospective lens cells from spreading. Continued cell proliferation within this restr  ...[more]

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