Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of gold and silicate nanoparticles

ABSTRACT: In this study, we investigated the gene expression induced by locally delivered gold and silicate nanoaprticles with the diameter of 20 and 100 nm in the retina. We injected nanoparticles into the vitreous cavity of 5-week-old male C57BL/6 mice. Au20 indicates gold nanoparticles of which diameters were 20 nm, Au100 gold nanoparticles of which diameters were 100 nm, Si20 silicate nanoparticles of which diameters were 20 nm, and Si100 silicate nanoparticles of which diameters were 100 nm.

ORGANISM(S): Mus musculus

PROVIDER: GSE49371 | GEO | 2013/07/31

SECONDARY ACCESSION(S): PRJNA213733

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of gold and silicate nanoparticles

Project description:In this study, we investigated the gene expression induced by locally delivered gold and silicate nanoaprticles with the diameter of 20 and 100 nm in the retina. We injected nanoparticles into the vitreous cavity of 5-week-old male C57BL/6 mice. Au20 indicates gold nanoparticles of which diameters were 20 nm, Au100 gold nanoparticles of which diameters were 100 nm, Si20 silicate nanoparticles of which diameters were 20 nm, and Si100 silicate nanoparticles of which diameters were 100 nm. We intravitreally injected PBS or nanoparticles (gold and silicate) into the right eyes of 5-week-old male C57BL/6 mice (n = 12 per group). PBS-treated mice were regarded as negative control. Four retinal tissues were pooled into 1 test tube and prepared for further analyses.

2013-07-31 | E-GEOD-49371 | biostudies-arrayexpress

STRT RNAseq of THP-1 monocytic cells exposed to surface modified-gold nanoparticles

Project description:Transcriptome comparison to assess the responses of human monocytic cells (THP-1) to gold-nanoparticles (Au-NPs) of two different diameter sizes (5 or 20 nm) with different surface functional groups, i.e., alkylammonium bromide, alkyl sodium carboxylate, or poly(ethylene glycol) (PEG)-terminated thiolate Au-NPs.

2019-06-04 | E-MTAB-7093 | biostudies-arrayexpress

Proteomics study of THP-1 monocytic cells exposed to surface modified-gold nanoparticles

Project description:A proteome comparison was performed to assess the responses of human monocytic cells (THP-1) to gold-nanoparticles (Au-NPs) of two different diameter sizes (5 or 20 nm) with different surface functional groups, i.e., alkylammonium bromide, alkyl sodium carboxylate, or poly(ethylene glycol) (PEG)-terminated thiolate Au-NPs.

2019-03-18 | PXD010858 | Pride

Understanding toxicity pathways of gold nanoparticles: RNA expression study on Caco-2 human colon carcinoma cells

Project description:A safer design and higher eficacy of nano-gold therapeutic formulations requires examination of cellular responses to gold nanoparticles (AuNPs). In this work we compared cellular uptake, cytotoxicity and RNA expression patterns induced in Caco-2 cells of citrate-stabilized Au NP of two different sizes (5 and 30 nm). The toxicity was measured with Colony Forming Efficiency (CFE) and Trypan Blue assays at 24 and 72 h after exposure to AuNPs in the 10 - 300 mM range. Toxicity was observed only in the CFE assay, at 200 and 300 mM exposure levels, and exclusively for smaller AuNPs size. Cellular internalization was dose and time-dependent for both AuNPs. The most pronounced changes in gene expression, evaluated with Agilent microarrays, were detected at 72 h (300 mM) for 5 nm AuNPs, with 103 up and 708 down regulated transcripts. For 30 nm AuNPs, only 4 gene transcripts were repressed under these conditions. The biological processes affected by 5 nm AuNPs were: RNA/zinc ion/transition metal ion binding (decreased), cadmium/copper ion binding and glutathione metabolism (increased). Some Nrf2 responsive genes (several metallothioneins, HMOX, G6PD, OSGIN1 and GPX2) were highly up regulated. Members of the selenoproteins (SELT, SELK, 15 kDa selenoprotein, SEPP1 and GPX2) were also differentially expressed. Our findings indicate that at high concentrations, smaller AuNPs can induce metal exposure and oxidative stress signaling pathways, and might influence selenium homeostasis. Therefore, some observed effects associated with nano-gold cytotoxicity can be further explored as potential enhancers of anti-cancer properties of already existing AuNPs based nanomedicin. Caco-2 cells were expoxed to spherical gold nanoparticles of two different sizes and concentrations (5 and 30 nm AuNPs, 100 and 300 microM). Cells were collected after 24 or 72 hours

2015-01-22 | E-GEOD-55349 | biostudies-arrayexpress

Nano gold simulated autophagy prevents cell death

Project description:We showed a kind of rectangular shaped gold-based nanoparticles (gold nanocages, GNC) could enhance the survival of cells from hydroxyurea induced cell apoptosis and significantly reduced the reactive oxygen species (ROS) levels.The nanoparticles could initiate single layer isolation membrane elongation after been phagocytosed, followed by autophagosome and autolysosome formation, then specifically integrated into the autophagosome inner membrane and bind to p62. Through recycling ribosome proteins and activate Nrf2 mediated activation of GATA3 signaling, rescue the cell death and enhanced cell and life survival.

2020-05-09 | GSE131033 | GEO

RNA SEQUENCING DATA OF NOVEL GOLD NANOPARTICLE TREATED PANCREATIC CANCER CELLS.

Project description:BxPC3 pancreatic cancer cell lines were treated with Gold Nanoparticles and RNA was isolated from untreated and treated cells, RNA was sequences by NGS method and data reported

2024-05-17 | GSE248677 | GEO

Understanding toxicity pathways of gold nanoparticles: RNA expression study on Caco-2 human colon carcinoma cells

2015-01-22 | GSE55349 | GEO

Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of titanium dioxide nanoparticles

Project description:In this study, we investigated the safety of locally delivered titanium dioxide nanoaprticles at the level of gene expression in the retina. To figure out any definite dose-dependent effect, we injected titanium dioxide nanoparticles into the vitreous cavity of 8-week-old male C57BL/6 mice at the concentration of presumptive therapeutic concentration (PTC; 130.47 ng/ml) and 10 times PTC (1.30 Î¼g/ml). We intravitreally injected PBS or titanium dioxide nanoparticles into the right eyes of 8-week-old male C57BL/6 mice (n = 12 per group). PBS-treated mice were regarded as negative control. Four retinal tissues were pooled into 1 test tube and prepared for further analyses.

2013-07-20 | E-GEOD-49048 | biostudies-arrayexpress

Transcriptomic response in gilthead sea bream (Sparus aurata) liver exposed to gold nanoparticles

Project description:Explore the underlying mechanisms-of-action after short-term (24 h) waterborne exposure to low (0.5 μg/L) and high (50 μg/L) gold nanoparticles (AuNP) concentrations in gilthead sea bream.

2017-01-24 | GSE93930 | GEO