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MethylC-Seq of idm2 single mutant and idm1idm2 double mutant

ABSTRACT: Using MethylC-Seq to provide single-base resolution of DNA methylation status in idm2 single mutant and idm1idm2 double mutant

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE49421 | GEO | 2014/07/10

SECONDARY ACCESSION(S): PRJNA213861

REPOSITORIES: GEO

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MethylC-Seq of idm2 single mutant and idm1idm2 double mutant

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2014-07-10 | E-GEOD-49421 | biostudies-arrayexpress

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MethlyC-Seq of Col-0 WT and mbd7-2(CS876032) mutant

Project description:In eukaryotes, heterochromatin is characterized by numerous epigenetic marks, including DNA methylation. Spreading of these marks into nearby active genes must be avoided in order to maintain appropriate gene expression. Here, we uncover Arabidopsis Methyl-CpG-Binding Domain 7 (MBD7) and Increased DNA Methylation 3 (IDM3) as anti-silencing factors that prevent transgene repression and genome-wide DNA hypermethylation. MBD7 preferentially binds to highly methylated, CG-dense regions associated with non-CG methylation and physically associates with other anti-silencing factors, including the histone acetyltransferase IDM1, IDM2, and IDM3. IDM1 and IDM2 were previously shown to facilitate active DNA demethylation by the 5-methylcytosine DNA glycosylase/lyase ROS1. Thus, MBD7 tethers the IDM proteins to methylated DNA, which enables the function of DNA demethylases that in turn establish chromatin boundaries and limit DNA methylation Using MethylC-Seq to provide single-base resolution of DNA methylation status in Col-0 WT and mbd7-2(CS876032) mutant

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