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MethylC-Seq of idm2 single mutant and idm1idm2 double mutant

ABSTRACT: Using MethylC-Seq to provide single-base resolution of DNA methylation status in idm2 single mutant and idm1idm2 double mutant MethylC-Seq: 2 mutants examined, idm2 single mutant (two biological replicates) and idm1idm2 double mutant

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jian-Kang Zhu

PROVIDER: E-GEOD-49421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of active DNA demethylation by an α-crystallin domain protein in Arabidopsis.

Qian Weiqiang W Miki Daisuke D Lei Mingguang M Zhu Xiaohong X Zhang Huiming H Liu Yunhua Y Li Yan Y Lang Zhaobo Z Wang Jing J Tang Kai K Liu Renyi R Zhu Jian-Kang JK

Molecular cell 20140704 3

DNA methylation patterns are dynamically controlled by DNA methylation and active DNA demethylation, but the mechanisms of regulation of active DNA demethylation are not well understood. Through forward genetic screens for Arabidopsis mutants showing DNA hypermethylation at specific loci and increased silencing of reporter genes, we identified IDM2 (increased DNA methylation 2) as a regulator of DNA demethylation and gene silencing. IDM2 dysfunction causes DNA hypermethylation and silencing of r ...[more]

PMID: 25002145

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