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Effects of DP1 knockdown on E2F target gene expression in normal human diploid fibroblasts.

ABSTRACT: Transcriptional profiling of normal human diploid fibroblasts(TIG-3) infected with retrovirous encoding DP1-shRNA or control-shRNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE49861 | GEO | 2014/04/10

SECONDARY ACCESSION(S): PRJNA215215

REPOSITORIES: GEO

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Effects of DP1 knockdown on E2F target gene expression in normal human diploid fibroblasts.

Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) infected with retrovirous encoding DP1-shRNA or control-shRNA. TIG-3 cells were subjected to infection with retrovirus encoding DP1-shRNA or control-shRNA. Total RNA was isolated using TRIzol reagent and were analyzed using the human 3D-Gene DNA chip (Toray) that contains 25000 genes. The genome wide transcriptional

2014-04-10 | E-GEOD-49861 | biostudies-arrayexpress

1D-DDA of human diploid fibroblasts, TIG-3 cells

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Gene expression profile of replicative senescence in normal human diploid fibroblasts.

Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) comparing late passage with early passage.

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Expression data from human fetal lung normal diploid fibroblasts

Project description:The interaction between cancer and stroma plays a key role in tumor progression. Inactivation of p53 is often observed in stromal cells surrounding in cancer, suggesting that p53 in fibroblasts is involved in tumor progression. To elucidate the mechanism by which p53 loss in fibroblasts effect on proliferation and invasiveness of cancer cells, we performed comprehensive expression profiling analyses between p53 knockdown and control fibroblasts using GeneChip Human Genome U133 plus 2.0 arrays. Intact human fetal lung normal diploid fibroblasts TIG-7 cells and TIG-7 cells infected with lentiviruses for the expression of shRNA against p53 were used for RNA extraction and hybridization on Affymetrix microarrays.

2014-08-01 | E-GEOD-58753 | biostudies-arrayexpress

Gene expression profile of oncogenic Ras-induced senescence in normal human diploid fibroblasts.

Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) comparing empty vector transducted cells with RasV12 transducted cells.

2014-04-10 | GSE49862 | GEO

RTNs and DP1 Knockdown in neurons

Project description:Quantitative proteomics results of RTNs-KD neurons or DP1-KD neurons (DIV5). Peptides derived from Untreated neurons (UNT) were chemically labeled with TMT-126, pSuper-neurons with TMT-127, shRNAs_RTNs-neurons with TMT-128 and shRNA_DP1-neurons with TMT-131.

2019-02-15 | PXD012264 | Pride

Gene expression profile of oncogenic Ras-induced senescence in normal human diploid fibroblasts.

Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) comparing empty vector transducted cells with RasV12 transducted cells. TIG-3 cells were subjected to infection with retrovirus encoding RasV12 or empty vector. Total RNA was isolated using TRIzol reagent and were analyzed using the human 3D-Gene DNA chip (Toray) that contains 25000 genes. The genome wide transcriptional resp

2014-04-10 | E-GEOD-49862 | biostudies-arrayexpress

Gene expression profile of p16-induced senescence in normal human diploid fibroblasts.

Project description:Transcriptional profiling of p16-induced senescent human diploid fibroblasts compared with proliferating cells. TIG-3 ER-p16 cells (primary normal human diploid fibroblasts expressing a 4-hydroxytamoxifen(4-OHT) regulatable form of human p16) were cultured for 7 days with or without 4-OHT. Total RNA was isolated using TRIzol reagent and were analyzed using the hum

2014-04-10 | E-GEOD-49863 | biostudies-arrayexpress

Gene expression profile of replicative senescence in normal human diploid fibroblasts.

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2014-04-10 | E-GEOD-49860 | biostudies-arrayexpress

ChIP-seq using a Dp1 antibody in Drosophila S2R+ cells

Project description:Here we report the identification of genomic regions of DNA bound by Dp1 in Drosophila S2R+ cells. Dp1 is a dimerization partner of several E2F transcription factors and is needed for E2F target promoter binding. We find that Dp1 binds the promoter regions of genes important for oxidative phosphorylation. This result is important since our data demonstrates that expression of several oxidative phosphorylation genes is down-regulated in dDP mutant Drosophila 3rd instar larval eye imaginal discs. These ChIP-seq results suggest that the mechanism by which dDP regulates expression of these genes is direct. In addition, we have confirmed a number of these Dp1 bound gene promoters by conventional Chromatin Immunoprecipitation.

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