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Transcriptomic analysis of early response of rat brain orthotopic tumors after synchrotron microbeam irradiation (MRT)

ABSTRACT: The aim of this study was to identify the transcriptomic response 6 hours after the MRT irradiation, in normal brain tissue (11 samples) and in glioma tissue (11 samples), in rat.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE50478 | GEO | 2015/09/01

SECONDARY ACCESSION(S): PRJNA217709

REPOSITORIES: GEO

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Transcriptomic analysis of early response of rat brain orthotopic tumors after synchrotron microbeam irradiation (MRT)

Project description:The aim of this study was to identify the transcriptomic response 6 hours after the MRT irradiation, in normal brain tissue (11 samples) and in glioma tissue (11 samples), in rat. The orthotopic tumor-bearing rats were either treated with MRT radiation (six MRT-tumors), or untreated (five No irradiated tumors). The contralateral half hemisphere without tumor received the MRT treatment for six rats (6 MRT-Contralateral samples) and no irradiation for five rats (5 Untreated contralateral samples). Six hours after treatment, the tumors and their controlateral regions were resected and analysed for transcriptomic response.

2015-09-01 | E-GEOD-50478 | biostudies-arrayexpress

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Time-dependent transcriptomic changes in HNSCC cell lines after irradiation with photons or Carbon ions

Project description:Purpose: We aim to identify irradiation-induced transciptional changes in HPV-positive and HPV-negative HNSCC cell lines in vitro. Methods: Cells were seeded to tissue culture flasks and irradiated after adherence, with 0 Gy or 4 Gy carbon ions or 4 Gy photons or 8 Gy photons. Analysis was performed 4h and 48h and 72h after irradiation Results: Analysis of the data reveals a clear difference between irradiated and unirradiated samples, as well as between HPV-positive and HPV-negative HNSCC cells Conclusion: Transcriptomic changes are dependent on HPV Status and irradiation, but independent from the type of irradiation.

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