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Effect of L-Proline on mouse embryonic stem cells (ESCs)

ABSTRACT: We found that the non-essential amino acid L-Proline (L-Pro) acts as a signaling molecule that promotes the conversion of embryonic stem cells (ESCs) into mesenchymal-like, spindle-shaped, highly motile, invasive pluripotent stem cells. This embryonic stem cell-to-mesenchymal-like transition (esMT) is accompanied by a genome-wide remodeling of the transcriptome We used microarrays to elucidate whether a diverse transcriptional program is the basis of the morphological and motility differences between ESCs and L- Proline treated ESCs (PiCs)

ORGANISM(S): Mus musculus

PROVIDER: GSE50603 | GEO | 2013/09/05

SECONDARY ACCESSION(S): PRJNA218064

REPOSITORIES: GEO

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Effect of L-Proline on mouse embryonic stem cells (ESCs)

Project description:We found that the non-essential amino acid L-Proline (L-Pro) acts as a signaling molecule that promotes the conversion of embryonic stem cells (ESCs) into mesenchymal-like, spindle-shaped, highly motile, invasive pluripotent stem cells. This embryonic stem cell-to-mesenchymal-like transition (esMT) is accompanied by a genome-wide remodeling of the transcriptome We used microarrays to elucidate whether a diverse transcriptional program is the basis of the morphological and motility differences between ESCs and L- Proline treated ESCs (PiCs) Total RNA was extracted from Control (ESCs) and L-Proline treated mouse embryonic stem cells (PiCs) and hybridized on Affimetrix microarrays.

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Mus musculus

Project description:Effect of L-Proline on mouse embryonic stem cells (ESCs)

| PRJNA217710 | ENA

Mus musculus

Project description:Effect of L-Proline on mouse embryonic stem cells (ESCs)

| PRJNA218064 | ENA

Expression profiling from mouse embryonic stem cells (ESCs)

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