Transcriptomics

Dataset Information

0

The effect of MPO inhibitor on the gene expression profiles in HL-60 cell lines exposed to 1, 2, 4,-benzenetriol


ABSTRACT: To reveal the gene expression profile changes after exposure to BT, one of benzene metabolites, with or without ABAH, a myeloperoxidase (MPO) inhibitor, and to elucidate how MPO is involved in myelotoxicity of BT, we performed microarray analysis. HL-60 cells suspended in RPMI 1640 containing with 10% of heat-inactivated FBS at 4×10^5/ mL were incubated with or without BT (50 μM) at 37℃ for one or 4 hours. For MPO inhibition experiment, HL-60 (4×10^5 cells/ mL) were pretreatment with 100 μM of ABAH in RPMI 1640/10% of heat-inactivated FBS at 37℃ for 24 hours. Media was then replaced with new media containing the regent plus BT (50 μM) and incubate at 37℃ for one or 4 hours. Unexposed HL-60 cells were used as controls. To validate the microarray results, we selected 22 genes and conducted a real-time PCR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE50805 | GEO | 2013/12/01

SECONDARY ACCESSION(S): PRJNA219057

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-12-01 | E-GEOD-50805 | biostudies-arrayexpress
2019-08-15 | GSE135818 | GEO
2009-12-31 | GSE19704 | GEO
2019-03-29 | PXD012052 | Pride
2013-04-24 | E-GEOD-46302 | biostudies-arrayexpress
2013-04-24 | GSE46302 | GEO
2022-01-19 | ST002061 | MetabolomicsWorkbench
2017-03-15 | GSE81005 | GEO
2017-03-15 | GSE81006 | GEO
2019-05-17 | E-MTAB-6439 | biostudies-arrayexpress