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Gcn2/WT polysome loading and transcript level in Arabidopsis thaliana

ABSTRACT: To understand the role of GCN2 in regulating translation, we compared the polysome loading state and overall transcript level between Arabidopsis thaliana wild type (ecotype Landsberg erecta) and gcn2 (Genetrap line GT8359, Cold Spring Harbor Laboratory) seedlings with or without herbicide chlorosufuron treatment RNA was fractionated using sucrose gradients into polysomal and nonpolysomal RNAs. We also determined overall total transcript levels. We used Affymetrix ATH1 microarrays.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE52116 | GEO | 2015/12/01

SECONDARY ACCESSION(S): PRJNA226718

REPOSITORIES: GEO

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