Variation in RNA-Seq transcriptome profiles of peripheral whole blood from healthy individuals with and without globin depletion
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ABSTRACT: In this study we aimed to assess technical variability associated with globin depletion in addition to assessing general technical variability in RNA-Seq from whole blood derived samples. We compared technical and biological replicates having undergone globin depletion or not and found that globin depletion removed approximately 80% of globin transcripts, improved the correlation of technical replicates, allowed for reliable detection of thousands of additional transcripts and generally increased transcript abundance measures.
ORGANISM(S): Homo sapiens
PROVIDER: GSE53655 | GEO | 2014/02/27
SECONDARY ACCESSION(S): PRJNA232593
REPOSITORIES: GEO
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