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Distinct functional programming of human fetal and adult monocytes [set 1]

ABSTRACT: Microarray profiling of unstimulated human fetal and adult bone marrow classical monocytes

ORGANISM(S): Homo sapiens

PROVIDER: GSE54668 | GEO | 2014/05/02

SECONDARY ACCESSION(S): PRJNA237317

REPOSITORIES: GEO

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Distinct functional programming of human fetal and adult monocytes [set 1]

Project description:Microarray profiling of unstimulated human fetal and adult bone marrow classical monocytes Classical monocytes were sort purified, RNA isolated, and amplified. 4 of each sample was collected and used

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Distinct functional programming of human fetal and adult monocytes [set 2]

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Distinct functional programming of human fetal and adult monocytes.

Project description:Preterm birth affects 1 out of 9 infants in the United States and is the leading cause of long-term neurologic handicap and infant mortality, accounting for 35% of all infant deaths in 2008. Although cytokines including interferon-γ (IFN-γ), interleukin-10 (IL-10), IL-6, and IL-1 are produced in response to in utero infection and are strongly associated with preterm labor, little is known about how human fetal immune cells respond to these cytokines. We demonstrate that fetal and adult CD14(+)CD16(-) classical monocytes are distinct in terms of basal transcriptional profiles and in phosphorylation of signal transducers and activators of transcription (STATs) in response to cytokines. Fetal monocytes phosphorylate canonical and noncanonical STATs and respond more strongly to IFN-γ, IL-6, and IL-4 than adult monocytes. We demonstrate a higher ratio of SOCS3 to IL-6 receptor in adult monocytes than in fetal monocytes, potentially explaining differences in STAT phosphorylation. Additionally, IFN-γ signaling results in upregulation of antigen presentation and costimulatory machinery in adult, but not fetal, monocytes. These findings represent the first evidence that primary human fetal and adult monocytes are functionally distinct, potentially explaining how these cells respond differentially to cytokines implicated in development, in utero infections, and the pathogenesis of preterm labor.

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Distinct functional programming of human fetal and adult monocytes

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Distinct functional programming of human fetal and adult monocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

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