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Expression and incorporation of microRNA in exosomes released by leukemia cells under room air and hypoxia

ABSTRACT: We recently showed that exosomes from primary AML cells and cell lines have potent regulatory capacity and we hypothesized that leukemia cell exosome trafficking might account for the suppression of residual hematopoietic stem and progenitor cells (HSPC) in the leukemic niche. Here we studied Molm-14 cells in in vitro experiments using purified exosomes under carefully calibrated low-oxygen conditions. This approach revealed the active regulation of stromal- and hematopoietic progenitor cell function. Systematic analysis of AML exosomes identified a panel of differentially enriched hypoxia-responsive miRNAs, including miR-210, -155, and -146a.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE55025 | GEO | 2014/02/20

SECONDARY ACCESSION(S): PRJNA238620

REPOSITORIES: GEO

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Expression and incorporation of microRNA in exosomes released by leukemia cells under room air and hypoxia

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