Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide analysis of cartilage gene expression in response to cartilage-specific ablation of Site-1 protease (Mbtps1) in mice (S1Pcko mice)

ABSTRACT: Analysis of differential gene expression in chondrocytes due to S1P ablation in mice. The hypothesis tested in this study was that ablation of S1P in mice would result in differential gene expression in chondrocytes that may be responsible for the S1Pcko phenotype.

ORGANISM(S): Mus musculus

PROVIDER: GSE55577 | GEO | 2014/07/31

SECONDARY ACCESSION(S): PRJNA240096

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide analysis of cartilage gene expression in response to cartilage-specific ablation of Site-1 protease (Mbtps1) in mice (S1Pcko mice)

Project description:Analysis of differential gene expression in chondrocytes due to S1P ablation in mice. The hypothesis tested in this study was that ablation of S1P in mice would result in differential gene expression in chondrocytes that may be responsible for the S1Pcko phenotype. The chondroepiphyseal cartilage from the tip of appendicular bones such as femur, tibia, humerus, radius, ulna were harvested from E16.5 wild type (WT) and S1Pcko embryos. In addition, the appendicular bones and calvarium from E16.5 WT and S1Pcko embryos also were harvested and processed for total RNA without removing the chondroepiphyseal cartilage. The chondroepiphyseal cartilage and bones were rolled on Whatman Filter paper to remove muscle and blood vessels, and total RNA was extracted, labeled, hybridized on Illumina Mouse WG-6 v1.1 and analyzed to identify genes that were differentially expressed in the S1Pcko skeletal elements when compared to WT.

2014-07-31 | E-GEOD-55577 | biostudies-arrayexpress

Global expression analysis of the transcriptome from bone marrow cells from wild type (WT) mice and mice with S1P-ablation in the Osterix-lineage (Cko)

Project description:Comparison of the transcriptome of non-RBC bone marrow cells from WT mice with non-RBC bone marrow cells from mice with S1P-ablation in the Osterix-lineage (Cko).

2020-06-04 | GSE146157 | GEO

High througuput sequencing of skeletal muscle-specific Site-1 Protease knockout mouse gastrocnemius and soleus

Project description:The processes by which mitochondria respond to cellular energy demands brought on by physiologic and pathophysiologic stimuli are essential to cellular adaptation and organismal survival. Disrupted mitochondrial function is implicated in several human disease states, underscoring the need to elucidate the molecular mechanisms that control mitochondrial function and metabolism. We previously described a patient with a gain-of-function mutation in Site-1 Protease (S1P) who exhibited idiopathic hyperCKemia, myoedema, and altered muscle mitochondrial morphology. To date, S1P has been heavily characterized in liver and bone, with very little known about its potential function in skeletal muscle. In our current study, we generated a skeletal muscle-specific S1P knockout mouse line (S1PsmKO) to examine the role of S1P in skeletal muscle. S1PsmKO mice were overtly normal in their appearance and health. However, examination of 12-week-old S1PsmKO mice showed increased gastrocnemius muscle mass and aged S1PsmKO mice exhibited increased muscle mass in both gastrocnemius and soleus relative to age-matched controls. Moreover, isolated muscle from S1PsmKO exhibited increased maximal mitochondrial respiration relative to floxed littermates (controls). RNA-Seq analysis showed increased transcript numbers of the mitochondrial-resident gene, Mss51 in S1PsmKO gastrocnemius relative to control mice. Mss51 is a member of the TGF-β/MSTN pathway, which controls mitochondrial metabolism. Similar to S1PsmKO muscle, S1P-depleted C2C12 cells also had increased maximal respiration, which was reversed by exogenous MSS51 expression. These data identify S1P as a regulator of muscle mass and mitochondrial metabolism, and implicate S1P as a novel modulator of TGF-β signaling. Method: Male mice were maintained on standard chow diet until 12 weeks of age.

2023-04-01 | GSE199014 | GEO

Ablation of the miRNA cluster 24 has profound effects on extracellular matrix protein abundance in cartilage

Project description:MicroRNAs (miRNAs) regulate cartilage differentiation and contribute to the onset and progression of joint degeneration. These small RNA molecules may affect extracellular matrix organization (ECM) in cartilage, but for only a few miRNAs has this role been defined in vivo. Previously, we showed that cartilage-specific genetic ablation of the Mirc24 cluster in mice leads to impaired cartilage development due to increased RAF/MEK/ERK pathway activation. Here, we studied the expression of the cluster in cartilage by LacZ reporter gene assays and determined its role for extracellular matrix homeostasis by proteome and immunoblot analysis. The cluster is expressed in prehypertrophic/hypertrophic chondrocytes of the growth plate and we now show that the cluster is also highly expressed in articular cartilage. Cartilage-specific loss of the cluster leads to increased proteoglycan 4 and matrix matrix metallopeptidase 13 levels and decreased aggrecan and collagen X levels in epiphyseal cartilage. Interestingly, these changes are linked to a decrease in SRY-related HMG box-containing (SOX) transcription factors 6 and 9, which regulate ECM production in chondrocytes. Our data suggests that the Mirc24 cluster is important for ECM homoeostasis and the expression of transcriptional regulators of matrix production in cartilage.

2020-06-18 | PXD019374 | Pride

Effect of adseverin deletion on gene expression in the articular cartilage of 10 week old mice

Project description:To investigate the alterations in the transcriptome profile due to adseverin deletion in articular chondrocytes, differential gene expression profiling analysis was performed using data obtained from RNA sequencing of articular chondrocytes in the native cartilage of 10 week old mice

2023-08-05 | GSE209578 | GEO

Targeting parkin-regulated metabolomic change in cartilage in the treatment of osteoarthritis

Project description:Articular cartilage degeneration, which may lead to osteoarthritis (OA), is a normal component of the aging process, but its underlying mechanism remains unknown. We found that chondrocytes exhibited an energy metabolism shift from glycolysis to oxidative phosphorylation (OXPHOS) during aging. Reprogrammed cartilage metabolism by parkin ablation decreased OXPHOS and increased glycolysis, with ameliorated aging-related OA. Metabolomics analysis indicated that lauroyl-L-carnitine (LLC) was decreased in aged cartilage, but increased in parkin-deficient cartilage. In vitro, LLC improved the cartilage matrix synthesis of OA chondrocytes. In vivo, intraarticular injection of LLC in mice with anterior cruciate ligament transaction (ACLT) ameliorated OA. These results suggest that metabolic changes are regulated by parkin-impaired cartilage during aging, and targeting the metabolomic changes by supplementation with LLC is a promising treatment strategy for ameliorating OA.

2024-07-17 | MTBLS9079 | MetaboLights

Targeting parkin-regulated metabolomic change in cartilage in the treatment of osteoarthritis

2024-07-17 | MTBLS9074 | MetaboLights

The global gene expression profile of superficial zone articular cartilage in P1 wildtype and Prg4 transgenic mice

Project description:To examine the unbiased global gene expression of over-expression of Prg4 in superficial zone articular chondrocytes in mice. This approach allows us to define novel signaling changes caused by the over-expression of Prg4 in superficial zone articular chondrocytes Total RNA was isolated from superficial zone chondrocytes in P1 articular cartilage in hind limbs (n=3) by laser capture microdissection. Same tissue from wild type mice served as control

2013-06-24 | E-GEOD-43663 | biostudies-arrayexpress

RNA Sequencing Analyzes Chondrocyte Transcriptomes of Wild-type, miR-26a -/- and Cartilage-specific miR-23a/b clusters Knockout Mice

Project description:To reveal the potential regulation target genes of miR-26a and miR-23a/b clusters in articular chondrocytes, we performed RNA-seq using RNA samples from cultured chondrocytes, which were primarily isolated from 3-week-old wild-type, miR-26a -/- or miR-23a/b cluster flox/flox;Col2a1-cre mice. Single-read libraries were prepared and sequenced by Illumina Nextseq 500. Proteins from the same sample were extracted for LC-MS/MS analysis. After data processing, differential expressed genes were screened out for exploring potential miRNA regulation targets.

2023-01-25 | GSE197363 | GEO