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Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

ABSTRACT: 8-cell stage rat embryos were sired by males treated with saline or a chemotherapeutic cocktail, BEP, consisting of Bleomycin, Etoposide, and Cisplatin for 9 weeks. Animals were given an additional 9 weeks of recovery, without any treatment, prior to mating.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE55842 | GEO | 2014/03/13

SECONDARY ACCESSION(S): PRJNA241124

REPOSITORIES: GEO

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Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

Project description:8-cell stage rat embryos were sired by males treated with saline or a chemotherapeutic cocktail, BEP, consisting of Bleomycin, Etoposide, and Cisplatin for 9 weeks. Animals were given an additional 9 weeks of recovery, without any treatment, prior to mating. qPCR gene expression profiling for 84 stem cell transcription factors. Total RNA extracted from 25-35 embryos at the eight-cell stage sired by control or BEP-treated males after a 9-wk recovery period (2 females mated per male, n= 4 males/treatment).

2014-03-13 | E-GEOD-55842 | biostudies-arrayexpress

Gene Expression Analysis of Postnatal Day 6 Testes Sired by Paternally Exposed Males to Saline or BEP

Project description:To determine the changes in gene expression of testes from postnatal day 6 pups, a window in development where spermatogonial stem cells is predominant, following paternal exposure to saline or BEP, a chemotherapeutic coktail used to treat testicular cancer Postnatal day 6 testes (2) of pups sired by males treated with saline (n=5) or BEP (n=5) for a 9 weeks followed by a 9-week recovery period

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Project description:Lung fibrosis represents the final common pathway of a variety of lung injuries, and it is characterized, independently of etiology, by the expansion of the fibroblast/myofibroblast population and by the abnormal accumulation of extracellular matrix (ECM) replacing normal functional parenchyma. In general, there is no effective therapy, and currently the only helpful available treatment for advanced lung fibrosis is transplantation. In this study we aimed to document the time-related reversibility of bleomycin-induced lung fibrosis, and to determine the gene expression patterns associated with the initial progression and the subsequent regression of the fibrotic response by microarray in this bleomycin mouse model of lung fibrosis. Mice (C57BL/6 ) aging 8 to 10 weeks were instilled intratracheally with 0.10 U/10 g of bleomycin (Blenoxane, Bristol-Myers Squibb Co.) in 50 μl sterile saline. Control animals received an equal volume of sterile saline (9, 10). Mice were housed in specific pathogen-free conditions and provided with food and water ad libitum. All studies and procedures were approved by the Science and Ethic Committee at the National Institute of Respiratory Diseases and were performed according to the protocols and guidelines of the institutional animal care and use committee. Mice were sacrificed at 7 days and 1, 2, 3, and 4 months after bleomycin or saline treatment. For every variable, six control and six experimental mice were used.

2013-03-11 | E-GEOD-42301 | biostudies-arrayexpress

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Expression data from mouse lung treated with saline or bleomycin

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Project description:Mice were given either water, an antibiotic cocktail (ANMV), or Neomycin in their drinking water for two weeks to reduce bacterial load, and then exposed to air or to ozone (2ppm for 3hrs). 24 hours later, pulmonary mechanics and airway responsiveness was assessed, and blood was collected via cardiac puncture. Serum was isolated and immediately stored at -80 C for further analysis.

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