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Detection of circRNA in Drosophila Fly heads and S2 cells using RNA-seq with or without RNaseR tretment

ABSTRACT: We determined circRNA abundance in fly Heads and S2 cells by generating and analyzing high-throughput RNA-sequencing libraries prepared from rRNA-depleted RNA. In order to determine whether the observed sequencing reads are due to bona fide circRNAs, we pre-treated the RNA with RNAse-R before the rRNA-depletion procedure. Indeed, most of the identified circRNAs were more enriched in comparison to the canonical mRNA isoforms following the RNAse-R treatment. We compare circRNA levels in wt (Canton S) flies with flies carrying the C4 ("slow polymerase") mutation.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE55872 | GEO | 2014/10/14

SECONDARY ACCESSION(S): PRJNA241181

REPOSITORIES: GEO

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Detection of circRNA in Drosophila Fly heads and S2 cells using RNA-seq with or without RNaseR tretment

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